Yu Myunghwan, Oketch Elijah Ogola, Nawarathne Shan Randima, Chathuranga Nuwan Chamara, Maniraguha Venuste, Sta Cruz Bernadette Gerpacio, Seo Eunsoo, Lee Jeseok, Park Haeeun, Choi Hyunji, Yi Young-Joo, Chae Kyeong Su, Yu Joosung, Heo Jung Min
Department of Animal Science and Biotechnology, Chungnam National University, Daejeon 34134, Republic of Korea.
Department of Agricultural Education, College of Education, Suncheon National University, Suncheon 57922, Republic of Korea.
Poult Sci. 2025 Apr 28;104(7):105222. doi: 10.1016/j.psj.2025.105222.
The objective of this study is to investigate the effect of β-mannanase in energy and amino acid-deficient broiler diets on the growth performance, carcass yields, ileal nutrient digestibility, viscosity, intestinal morphology, and blood metabolites of broilers from hatch to day 35. A total of 168 one-day-old Ross 308 broiler chicks were randomly assigned to one of three dietary treatments in a completely randomized design, with each treatment having eight replicates and seven birds per cage. The treatments were as follows: i) positive control diet (PC) containing standard energy and amino acids; ii) negative control (NC) with 150 kcal/kg metabolizable energy (ME) and 4.5 % amino acids reduction compared to PC; iii) NC supplemented with 100 g/ton β-mannanase (NC + β-mannanase). Broilers fed the PC and NC + β-mannanase diets showed improved growth performance, including body weight on day 35 (P < 0.001), average daily gain (Day 1-35; P = 0.001), and feed efficiency (Day 1-35; P < 0.001), alongside lower (P < 0.001) digesta viscosity and enhanced ileal digestibility of dry matter (P < 0.001), crude protein (P < 0.001), and energy (P < 0.001), as well as an improved (P < 0.001) villus height to crypt depth ratio, compared to those on the NC diet. Regarding carcass traits, liver yield was higher (P < 0.001) in broilers on the NC + β-mannanase diet compared to other treatments by day 35. Additionally, the NC + β-mannanase diet lowered (P < 0.001) IL-1β and IFN-γ levels and increased (P < 0.001) IL-10 levels compared to the other groups. In conclusion, β-mannanase supplementation with 100 g/MT β-mannanase in nutrient-deficient diets improved broiler performance by reducing intestinal viscosity and enhancing nutrient utilization efficiency. It also promoted the development of metabolic organs like the liver, as well as immune organs such as the spleen and thymus, while modulating inflammatory and anti-inflammatory cytokine responses.
本研究的目的是调查β-甘露聚糖酶对能量和氨基酸缺乏的肉鸡日粮从孵化到35日龄的肉鸡生长性能、胴体产量、回肠养分消化率、粘度、肠道形态及血液代谢物的影响。总共168只1日龄罗斯308肉鸡雏鸡被完全随机设计分配到三种日粮处理中的一种，每种处理有8个重复，每个笼子7只鸡。处理如下：i）含标准能量和氨基酸的阳性对照日粮（PC）；ii）与PC相比，代谢能（ME）降低150 kcal/kg且氨基酸降低4.5%的阴性对照（NC）；iii）添加100 g/吨β-甘露聚糖酶的NC（NC + β-甘露聚糖酶）。与NC日粮组相比，饲喂PC和NC + β-甘露聚糖酶日粮的肉鸡生长性能得到改善，包括35日龄体重（P < 0.001）、平均日增重（第1 - 35天；P = 0.001）和饲料效率（第1 - 35天；P < 0.001），同时消化物粘度更低（P < 0.001），干物质（P < 0.001）、粗蛋白（P < 0.001）和能量（P < 0.001）的回肠消化率提高，绒毛高度与隐窝深度比值改善（P < 0.001）。关于胴体性状，到35日龄时，NC + β-甘露聚糖酶日粮组肉鸡的肝脏产量高于其他处理组（P < 0.001）。此外，与其他组相比，NC + β-甘露聚糖酶日粮降低了（P < 0.001）IL - 1β和IFN - γ水平，提高了（P < 0.001）IL - 10水平。总之，在营养缺乏的日粮中添加100 g/MT β-甘露聚糖酶可通过降低肠道粘度和提高养分利用效率来改善肉鸡性能。它还促进了肝脏等代谢器官以及脾脏和胸腺等免疫器官的发育，同时调节炎症和抗炎细胞因子反应。
