Ireland P A, Dziedzic S Z
J Chromatogr. 1985 May 29;325(1):275-81. doi: 10.1016/s0021-9673(00)96028-x.
A method for the analysis of soybean sapogenins is described. The method is based on the extraction of soybean saponins from a defatted sample. The triterpene glycosides are then hydrolysed with subsequent analysis of the liberated sapogenins by high-performance liquid chromatography using gradient elution and mass detection. By use of a sapogenin/carbohydrate ratio, an estimate of the total saponin content can be made.
描述了一种分析大豆皂草苷元的方法。该方法基于从脱脂样品中提取大豆皂苷。然后将三萜糖苷水解,随后通过使用梯度洗脱和质谱检测的高效液相色谱法对释放的皂草苷元进行分析。通过使用皂草苷元/碳水化合物比率,可以估算总皂苷含量。