Rao D, Bories G
Laboratoire des Xénobiotiques, I.N.R.A., Toulouse, France.
J Chromatogr. 1987 Nov 20;410(1):169-75. doi: 10.1016/s0021-9673(00)90044-x.
A gas chromatographic method for the determination of medicagenic acid in alfalfa leaves, stems, entire plant (tops) and roots and also in leaf protein concentrates is described. The method is based on extraction of lucerne saponins followed by hydrolysis of the triterpene glycosides. After derivatization of the liberated sapogenins to silylated products, the trimethylsilylated medicagenic acid was determined by gas chromatography. The sensitivity of the method permits the detection of 50 ng of medicagenic acid.
本文描述了一种气相色谱法，用于测定苜蓿叶、茎、全株（顶部）、根以及叶蛋白浓缩物中的苜蓿酸。该方法基于对苜蓿皂苷的提取，随后对三萜糖苷进行水解。将游离的皂苷元衍生化为硅烷化产物后，通过气相色谱法测定三甲基硅烷化的苜蓿酸。该方法的灵敏度可检测到50纳克的苜蓿酸。
