Brook J D, Chandley A C
Mutat Res. 1985 Aug-Sep;157(2-3):215-20. doi: 10.1016/0165-1218(85)90118-1.
The 3 chemicals, 6 mercaptopurine (6-MCP), phenylalanine and para-fluorophenylalanine (pFPA) have been tested on mouse oocytes of the Swiss strain for possible aneuploidy-inducing effects. Tests were made at the dictyate stage in young and aged females and at the preovulatory (diakinesis/MI) stage in aged females only. Metaphase II chromosome complements were analysed for aneuploidy resulting from segregational errors arising at the first meiotic division. No evidence of non-disjunction was found either in treated or control groups up to the age of 40 weeks tested. The need to select for gametogenic stage and strain when using a mouse model system for aneuploidy testing, is considered.
已对三种化学物质，即6-巯基嘌呤（6-MCP）、苯丙氨酸和对氟苯丙氨酸（pFPA）在瑞士品系小鼠卵母细胞上进行了测试，以研究其可能的非整倍体诱导作用。测试在年轻和老龄雌性小鼠的双线期以及仅在老龄雌性小鼠的排卵前期（终变期/减数分裂Ⅰ期）进行。分析了中期Ⅱ染色体组型，以检测第一次减数分裂时发生分离错误导致的非整倍体情况。在所测试的40周龄以内的处理组和对照组中，均未发现不分离的证据。文中还考虑了在使用小鼠模型系统进行非整倍体测试时选择配子发生阶段和品系的必要性。
