Antunes-Ferreira Mafalda, Glogovitis Ilias, Fortunato Diogo, D'Ambrosi Silvia, Saborit Mariona Colom, Yahubyan Galina, Baev Vesselin, Hackenberg Michael, Zarovni Natasa, Wurdinger Thomas, Koppers-Lalic Danijela
Cancer Center Amsterdam, Amsterdam University Medical Centers, 1081 HV Amsterdam, The Netherlands.
Department of Molecular Biology, University of Plovdiv, Tzar Assen 24, 4000 Plovdiv, Bulgaria.
Noncoding RNA. 2025 May 7;11(3):38. doi: 10.3390/ncrna11030038.
Platelet-derived Extracellular Vesicles, or "Platelet Dust" (PD), are reported as the most-abundant extracellular vesicles in plasma. However, the PD molecular content, especially the small RNA profile, is still poorly characterized. This study aims to characterize PD and other extracellular vesicles (EVs) in patients with non-small-cell lung cancer (NSCLC), focusing on their small RNA signatures and diagnostic potential. The EVs were isolated directly from the plasma of healthy donors and patients with NSCLC using the surface markers CD9, CD63, CD81 (overall EVs), and CD61 (PD). Small RNA sequencing was then performed to comprehensively profile the miRNAs. Our analysis revealed distinct small RNA profiles in the EVs and the PD from the patients with NSCLC. The EVs (CD9-, CD63-, and CD81-positive) showed the enrichment of four miRNAs and the depletion of ten miRNAs, while the PD (CD61-positive) exhibited a more complex profile, with nineteen miRNAs enriched and nine miRNAs depleted in the patients with NSCLC compared to those of the healthy controls. This exploratory study enhances our understanding of miRNA composition within different plasma vesicle populations, shedding light on the biology of plasma vesicles and their contents. Furthermore, utilizing an extracellular vesicle isolation method with potential clinical applicability offers the prospect of improved cancer characterization and detection by selecting the most informative subpopulation of plasma vesicles.
血小板衍生的细胞外囊泡，即“血小板尘埃”（PD），据报道是血浆中最丰富的细胞外囊泡。然而，PD的分子含量，尤其是小RNA谱，仍未得到充分表征。本研究旨在表征非小细胞肺癌（NSCLC）患者的PD和其他细胞外囊泡（EVs），重点关注其小RNA特征和诊断潜力。使用表面标志物CD9、CD63、CD81（总体EVs）和CD61（PD）直接从健康供体和NSCLC患者的血浆中分离EVs。然后进行小RNA测序以全面分析miRNA。我们的分析揭示了NSCLC患者的EVs和PD中不同的小RNA谱。EVs（CD9、CD63和CD81阳性）显示四种miRNA富集，十种miRNA耗竭，而PD（CD61阳性）表现出更复杂的谱，与健康对照相比，NSCLC患者中有19种miRNA富集，9种miRNA耗竭。这项探索性研究增进了我们对不同血浆囊泡群体中miRNA组成的理解，阐明了血浆囊泡及其内容物的生物学特性。此外，利用具有潜在临床适用性的细胞外囊泡分离方法，通过选择最具信息性的血浆囊泡亚群，有望改善癌症的表征和检测。
