Sarwar G, Peace R W, Botting H G
J Assoc Off Anal Chem. 1985 Jul-Aug;68(4):689-93.
The requirement for sulfur amino acids was calculated for growing rats fed 8% protein diets. The rat and human requirements for sulfur amino acids were compared and a correction factor was developed to reflect the differences. This correction factor was used to determine corrected relative net protein ratio (CRNPR) values for a number of potential meat extenders and their mixtures with beef. The methionine + cystine requirement of growing rats was estimated to be 4% of protein for 8% protein diets. The methionine + cystine requirement of rats was about 50% higher than that of humans (2.65% of protein). Based on this comparison (rat/human), a correction factor of 1.5 was developed to correct RNPR values of those protein products that were deficient in sulfur amino acids for rat growth. The CRNPR values of beef, casein, soybean protein products, pea concentrate, and peanut meal were 100, 100, 91-97, 75, and 73, respectively. Mixtures (50:50 protein basis) of beef with casein, soybean concentrate, soybean isolate, pea concentrate, peanut meal, rapeseed concentrate, rapeseed isolate, sunflower isolate, or wheat gluten were equal to beef in CRNPR values. The CRNPR method is a good predictor of protein quality for humans of those protein products that are deficient in sulfur amino acids.
针对食用8%蛋白质日粮的生长大鼠，计算了其对含硫氨基酸的需求量。比较了大鼠和人类对含硫氨基酸的需求量，并制定了一个校正因子以反映两者之间的差异。该校正因子用于确定多种潜在肉类增量剂及其与牛肉混合物的校正相对净蛋白比（CRNPR）值。对于8%蛋白质日粮，生长大鼠的蛋氨酸+胱氨酸需求量估计为蛋白质的4%。大鼠的蛋氨酸+胱氨酸需求量比人类高约50%（占蛋白质的2.65%）。基于这种比较（大鼠/人类），制定了一个1.5的校正因子，用于校正那些硫氨基酸含量不足、不利于大鼠生长的蛋白质产品的RNPR值。牛肉、酪蛋白、大豆蛋白产品、豌豆浓缩物和花生粕的CRNPR值分别为100、100、91 - 97、75和73。牛肉与酪蛋白、大豆浓缩物、大豆分离物、豌豆浓缩物、花生粕、菜籽浓缩物、菜籽分离物、向日葵分离物或小麦面筋以蛋白质计50:50的混合物，其CRNPR值与牛肉相当。对于那些硫氨基酸含量不足的蛋白质产品，CRNPR方法是预测其对人类蛋白质质量的良好指标。
