Das Bini C, Pillai Devika, V J Rejish Kumar
Department of Aquatic Animal Health Management, Kerala University of Fisheries and Ocean Studies, Kochi, Kerala, India.
Department of Aquaculture, Kerala University of Fisheries and Ocean Studies, Kochi, Kerala, India.
Aquat Toxicol. 2025 Aug;285:107418. doi: 10.1016/j.aquatox.2025.107418. Epub 2025 May 18.
Nanoplastics, as widespread and persistent pollutants, pose a significant threat to the health of organisms. In this study, the impacts of polystyrene nanoplastics on the euryhaline fish, pearl spot (Etroplus suratensis) were investigated. Pearl spot were exposed to 0, 0.2, 2, and 4 mg/L of PS NPs for 14 days and NP accumulation, as well as its impact of accumulation on biochemical and oxidative stress parameters and gene expression were examined. The NP accumulation pattern was different at different exposure concentrations. At 0.2 mg/L of NP exposure concentration, the accumulation order observed was gills ˃ liver ˃ muscle ˃ intestine ˃ brain ˃ spleen. However, at higher exposure concentrations (2 and 4 mg/L), the accumulation order changed to intestine ˃ liver ˃ gills ˃ muscle ˃ spleen ˃ brain. Moreover, the accumulation led to considerable variations in biochemical parameters. Glucose, total cholesterol, SGOT, SGPT, and ALP levels increased, while albumin, total protein, and A/G ratio decreased due to NP exposure. Additionally, the antioxidant levels including SOD, CAT, GPx, and TAC, were remarkably reduced. This could explain the higher levels of MDA and PC, as well as the reduced expression of NRF2 and P53 in the NP-exposed groups, indicating oxidative damage. The significant increases in cortisol levels and the up-regulation of HSP70 indicate that fish experience stress evoked by NP. The NP exposure reduced the IGF1 and CYP1A expression, indicating its potential to impair growth and xenobiotic metabolism. These findings indicate that NPs induce stress, biochemical changes, oxidative damage, inhibited growth, and metabolism disruption in fish. This study is the first to examine the environmentally relevant NPs concentrations on protein damage from oxidative stress, toxic metabolism, and the expression of NRF2, P53, IGF1, and CYP1A in a Cichlid fish.
纳米塑料作为广泛存在且持久的污染物，对生物体健康构成重大威胁。在本研究中，调查了聚苯乙烯纳米塑料对广盐性鱼类珍珠斑（Etroplus suratensis）的影响。将珍珠斑暴露于0、0.2、2和4 mg/L的聚苯乙烯纳米颗粒中14天，并检测纳米颗粒的积累情况，以及积累对生化和氧化应激参数及基因表达的影响。不同暴露浓度下纳米颗粒的积累模式不同。在0.2 mg/L的纳米颗粒暴露浓度下，观察到的积累顺序为鳃＞肝脏＞肌肉＞肠道＞大脑＞脾脏。然而，在较高暴露浓度（2和 4 mg/L）下，积累顺序变为肠道＞肝脏＞鳃＞肌肉＞脾脏＞大脑。此外，积累导致生化参数出现显著变化。由于纳米颗粒暴露，葡萄糖、总胆固醇、谷草转氨酶、谷丙转氨酶和碱性磷酸酶水平升高，而白蛋白、总蛋白和白球比降低。此外，包括超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶和总抗氧化能力在内的抗氧化剂水平显著降低。这可以解释纳米颗粒暴露组中丙二醛和蛋白质羰基水平较高，以及核因子E2相关因子2和P53表达降低的原因，表明存在氧化损伤。皮质醇水平的显著升高和热休克蛋白70的上调表明鱼类经历了纳米颗粒引起的应激。纳米颗粒暴露降低了胰岛素样生长因子1和细胞色素P450 1A的表达，表明其有损害生长和外源性物质代谢的潜力。这些发现表明，纳米颗粒会在鱼类中诱导应激、生化变化、氧化损伤、生长抑制和代谢紊乱。本研究首次考察了环境相关浓度的纳米颗粒对丽鱼科鱼类氧化应激导致蛋白质损伤、毒性代谢以及核因子E2相关因子2、P53、胰岛素样生长因子1和细胞色素P450 1A表达的影响。
