Lahti A, Maibach H I
J Am Acad Dermatol. 1985 Jul;13(1):66-9. doi: 10.1016/s0190-9622(85)70144-2.
In order to find the most suitable animal species for studies on nonimmunologic contact urticaria (NICU), human NICU agents: 20% benzoic acid (BA), 10% sorbic acid (SA), 15% cinnamic acid (CA), 20% cinnamic aldehyde (CAL), 1.0% diethyl fumarate (DEF), 0.2% methyl nicotinate (MN), all in absolute ethyl alcohol, and 100% dimethyl sulfoxide (DMSO) were each applied to the earlobes of guinea pigs, rats, and mice. Guinea pig ear reacted with swelling to all agents tested. The mean maximal increase in thickness of the guinea pig ear, measured with a micrometer, was 114 +/- 46%. No response to BA, SA, CA, DEF, or MN was noted in the rat or mouse ear. However, DMSO and CAL caused significant ear swelling both in the mouse and the rat. Thus, the guinea pig ear is more sensitive to a variety of human NICU agents than is rat or mouse ear. The striking differences in species reactivity to NICU agents suggest the possibility that there are several mechanisms (mediators) involved in reactions to different substances. The guinea pig ear swelling test remains the best quantitative animal method available for screening human NICU agents.
为了找到最适合用于非免疫性接触性荨麻疹（NICU）研究的动物物种，将人类NICU制剂：20%苯甲酸（BA）、10%山梨酸（SA）、15%肉桂酸（CA）、20%肉桂醛（CAL）、1.0%富马酸二乙酯（DEF）、0.2%烟酸甲酯（MN）（均溶于无水乙醇）以及100%二甲基亚砜（DMSO）分别涂抹于豚鼠、大鼠和小鼠的耳垂。豚鼠的耳朵对所有测试制剂均出现肿胀反应。用测微计测量，豚鼠耳朵厚度的平均最大增加量为114±46%。在大鼠或小鼠的耳朵上未观察到对BA、SA、CA、DEF或MN的反应。然而，DMSO和CAL在小鼠和大鼠耳朵上均引起了明显的肿胀。因此，豚鼠的耳朵对多种人类NICU制剂比大鼠或小鼠的耳朵更敏感。不同物种对NICU制剂反应性的显著差异表明，对不同物质的反应可能涉及多种机制（介质）。豚鼠耳朵肿胀试验仍然是用于筛选人类NICU制剂的最佳定量动物方法。
