Ohnishi S T
Membr Biochem. 1985;6(1):33-47. doi: 10.3109/09687688509065441.
Heavy sarcoplasmic reticulum (SR) was prepared from skeletal muscle of control and chronic alcoholic rats, and the effect of in vitro addition of ethanol on the passive Ca2+ permeability was studied. The SR was loaded with Ca2+ in the absence of ATP. Then efflux was initiated by adding an EGTA solution to decrease the extravesicular Ca2+ concentration. The decrease of Ca2+ content of the SR was measured by an optical method using an encapsulated metallochromic indicator (calcein). The Ca2+ permeability of alcoholic rat SR was higher than that of control rats, especially at low external Ca2+ concentrations (below 1 microM). An in vitro (acute) exposure of SR to ethanol increased the Ca2+ permeability of the SR. However, the degree of increase in alcoholic rat SR was smaller than that in control rat SR.
从对照大鼠和慢性酒精中毒大鼠的骨骼肌中制备了重肌质网（SR），并研究了体外添加乙醇对被动Ca2+通透性的影响。在无ATP的情况下，将SR用Ca2+加载。然后通过添加乙二醇双（2-氨基乙基醚）四乙酸（EGTA）溶液以降低囊泡外Ca2+浓度来启动外流。使用封装的金属显色指示剂（钙黄绿素）通过光学方法测量SR中Ca2+含量的降低。酒精中毒大鼠SR的Ca2+通透性高于对照大鼠，尤其是在低细胞外Ca2+浓度（低于1 microM）时。SR在体外（急性）暴露于乙醇会增加SR的Ca2+通透性。然而，酒精中毒大鼠SR的增加程度小于对照大鼠SR。
