Fuhrmann Falko, Nebeling Felix C, Musacchio Fabrizio, Mittag Manuel, Poll Stefanie, Müller Monika, Ambrad Giovannetti Eleonora, Maibach Michael, Schaffran Barbara, Burnside Emily, Chan Ivy Chi Wai, Lagurin Alex Simon, Reichenbach Nicole, Kaushalya Sanjeev, Fried Hans, Linden Stefan, Petzold Gabor C, Tavosanis Gaia, Bradke Frank, Fuhrmann Martin
Neuroimmunology and Imaging Group, German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany.
Department of Neurooncology, Center for Neurology, University Hospital Bonn, Bonn, Germany.
Commun Biol. 2025 May 23;8(1):795. doi: 10.1038/s42003-025-08079-8.
The medial prefrontal cortex (mPFC) is important for higher cognitive functions, including working memory, decision making, and emotional control. In vivo recordings of neuronal activity in the mPFC have been achieved via invasive electrical and optical approaches. Here we apply low invasive three-photon in vivo imaging in the mPFC of the mouse at unprecedented depth. Specifically, we measure neuronal and astrocytic Ca-transient parameters in awake head-fixed mice up to a depth of 1700 µm. Furthermore, we longitudinally record dendritic spine density (0.41 ± 0.07 µm) deeper than 1 mm for a week. Using 1650 nm wavelength to excite red fluorescent microglia, we quantify their processes' motility (58.9 ± 2% turnover rate) at previously unreachable depths (1100 µm). We establish three-photon imaging of the mPFC enabling neuronal and glial recordings with subcellular resolution that will pave the way for novel discoveries in this brain region.
内侧前额叶皮质（mPFC）对于包括工作记忆、决策和情绪控制在内的高级认知功能至关重要。通过侵入性电和光学方法已实现对mPFC中神经元活动的体内记录。在此，我们以前所未有的深度在小鼠的mPFC中应用低侵入性三光子体内成像。具体而言，我们在清醒的头部固定小鼠中测量深度达1700 µm的神经元和星形胶质细胞的钙瞬变参数。此外，我们纵向记录了深度超过1 mm的树突棘密度（0.41±0.07 µm），持续一周。使用1650 nm波长激发红色荧光小胶质细胞，我们在以前无法到达的深度（1100 µm）量化了它们突起的运动性（周转率为58.9±2%）。我们建立了mPFC的三光子成像，能够以亚细胞分辨率进行神经元和神经胶质记录，这将为该脑区的新发现铺平道路。
