Wang Qifeng, Zhao Yuyi, Li Shikuo, Li Xuehan, Wang Haiyan, Zuo Yunxia
Department of Anesthesiology, West China Hospital, Sichuan University, Chengdu, China.
Laboratory of Mitochondria and Metabolism, National-Local Joint Engineering Research Centre of Translational Medicine of Anesthesiology, West China Hospital, Sichuan University, Chengdu, China.
BMC Anesthesiol. 2025 May 24;25(1):265. doi: 10.1186/s12871-025-03140-9.
This study explored the association of surgical traumatic stress with volatile organic compounds (VOCs) in breath.
Exhaled breath and blood samples were collected from 105 patients under general anesthesia at three time points: prior to incision (Pre-op), 2 h after incision (Intra-op), and prior to extubation (End-op). Differential mass spectral features between these time points were screened. Traumatic stress-related biomarker detection and mixed-effects model analysis were performed to define correlations and significance between parameters. Subgroup analysis was conducted to test the ability of mass spectral features to distinguish different surgical routes (open vs. laparoscopic).
The abundances of mass spectral features changed significantly between these time points. All stress-related biomarker increased dramatically in the Intra-op group and decreased significantly after operation. Mixed-effects model analysis revealed that several features were significantly correlated with stress biomarkers. Most importantly, subgroup analysis revealed that different surgical routes failed to be distinguished by mass spectral features.
Surgical traumatic stress may change the exhaled breath mass spectral features in perioperative patients, providing preliminary evidence for mass spectral features use in future monitoring of this stress response.
本研究探讨手术创伤应激与呼出气体中挥发性有机化合物（VOCs）之间的关联。
收集105例全身麻醉患者在三个时间点的呼出气体和血液样本：切口前（术前）、切口后2小时（术中）和拔管前（术终）。筛选这些时间点之间的差异质谱特征。进行创伤应激相关生物标志物检测和混合效应模型分析，以确定参数之间的相关性和显著性。进行亚组分析，以测试质谱特征区分不同手术途径（开放手术与腹腔镜手术）的能力。
这些时间点之间的质谱特征丰度有显著变化。所有应激相关生物标志物在术中组显著增加，术后显著下降。混合效应模型分析显示，几个特征与应激生物标志物显著相关。最重要的是，亚组分析显示，质谱特征无法区分不同的手术途径。
手术创伤应激可能会改变围手术期患者呼出气体的质谱特征，为未来利用质谱特征监测这种应激反应提供了初步证据。
