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由大肠杆菌核糖体23 - S RNA和核糖体蛋白L24形成的复合物的核糖核蛋白片段中的RNA序列。

RNA sequences in ribonucleoprotein fragments of the complex formed from ribosomal 23-S RNA and ribosomal protein L24 of Escherichia coli.

作者信息

Branlant C, Sri Widada J, Krol A, Ebel J P

出版信息

Eur J Biochem. 1977 Mar 15;74(1):155-70. doi: 10.1111/j.1432-1033.1977.tb11377.x.

DOI:10.1111/j.1432-1033.1977.tb11377.x
PMID:404143
Abstract

Upon digestion of the complex formed from the 23-S ribosomal RNA and the 50-S ribosomal protein L24 of Escherichia coli, two fragments resistant to ribonuclease were recovered; these fragments contained RNA sections belonging to the 480 nucleotides at the 5' end of 23-S RNA. By determining the sequence of 70% of this latter region we were able to localise the sections which, in the presence of the protein, are resistant to ribonuclease. Our results suggest that the region encompassing the 480 nucleotides starting at the 9th nucleotide from the 5' end of 23-S RNA has a compact tertiary structure, which is stabilised by protein L24.

摘要

在消化由大肠杆菌的23 - S核糖体RNA和50 - S核糖体蛋白L24形成的复合物后，回收了两个对核糖核酸酶有抗性的片段；这些片段包含属于23 - S RNA 5'端480个核苷酸的RNA部分。通过确定后一个区域70%的序列，我们能够定位在蛋白质存在下对核糖核酸酶有抗性的部分。我们的结果表明，从23 - S RNA 5'端第9个核苷酸开始的包含480个核苷酸的区域具有紧密的三级结构，该结构由蛋白L24稳定。

相似文献

1
RNA sequences in ribonucleoprotein fragments of the complex formed from ribosomal 23-S RNA and ribosomal protein L24 of Escherichia coli.由大肠杆菌核糖体23 - S RNA和核糖体蛋白L24形成的复合物的核糖核蛋白片段中的RNA序列。
Eur J Biochem. 1977 Mar 15;74(1):155-70. doi: 10.1111/j.1432-1033.1977.tb11377.x.
2
The binding site of protein L1 ON 23-S ribosomal RNA of Escherichia coli. 2. Identification of the rna region contained in the L1 ribonucleoproteins and determination of the order of the RNA subfragments within this region.蛋白质L1与大肠杆菌23 - S核糖体RNA的结合位点。2. L1核糖核蛋白中所含RNA区域的鉴定以及该区域内RNA亚片段顺序的确定。
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RNA sequences associated with proteins L1, L9, and L5, L18, L25, in ribonucleoprotein fragments isolated from the 50-S subunit of Escherichia coli ribosomes.从大肠杆菌核糖体50-S亚基分离出的核糖核蛋白片段中，与蛋白质L1、L9、L5、L18、L25相关的RNA序列。
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Nucleotide sequences of the T1 and pancreatic ribonuclease digestion products from some large fragments of the 23S RNA of Escherichia coli.来自大肠杆菌23S RNA一些大片段的T1和胰核糖核酸酶消化产物的核苷酸序列。
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The determination of the primary structure of the 16S ribosomal RNA of Escherichia coli. III. Further studies.大肠杆菌16S核糖体RNA一级结构的测定。III. 进一步的研究。
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Eur J Biochem. 1976 Nov 1;70(1):159-70. doi: 10.1111/j.1432-1033.1976.tb10966.x.

引用本文的文献

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2
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J Bacteriol. 1980 Jan;141(1):365-73. doi: 10.1128/jb.141.1.365-373.1980.
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Nucleic Acids Res. 1984 Aug 10;12(15):6197-220. doi: 10.1093/nar/12.15.6197.
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EMBO J. 1986 Jun;5(6):1373-6. doi: 10.1002/j.1460-2075.1986.tb04369.x.
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