Branlant C, Widada J S, Krol A, Fellner P, Ebel J P
Biochimie. 1975;57(2):175-225. doi: 10.1016/s0300-9084(75)80168-4.
When the 23S RNA from E. Coli was pretreated for 1 h at 60 degrees in the presence of Mg++ and K+ and then subjected to T1 ribonuclease attack, resistant fragments were recovered from 3 regions of the molecule: region A (containing 470-500 nucleotides) located at the 5' end of 23S RNA, region B (containing 520-550 nucleotides) located at the 3' end and region C (containing 110-120 nucleotides) lying between region A and region B. The nucleotide sequences of the T1 and pancreatic ribonuclease digestion products from these 3 regions have been studied and in most cases determined. In the course of these studies, a certain number of abnormal nucleotides, which are not methylated, have been encountered. A low level of sequence heterogeneity was detected.
当来自大肠杆菌的23S RNA在Mg++和K+存在的条件下于60摄氏度预处理1小时，然后用T1核糖核酸酶攻击时，从该分子的3个区域回收了抗性片段：区域A（包含470 - 500个核苷酸）位于23S RNA的5'端，区域B（包含520 - 550个核苷酸）位于3'端，区域C（包含110 - 120个核苷酸）位于区域A和区域B之间。已经研究并在大多数情况下确定了来自这3个区域的T1和胰核糖核酸酶消化产物的核苷酸序列。在这些研究过程中，遇到了一定数量未甲基化的异常核苷酸。检测到了低水平的序列异质性。
