Jayakody Nishani K, Klug Christopher A, Bovee Mark O, Smith Aaron D, Dysart Jennifer L, Laskoski Matthew, Glaser Evan R, Hennessa Tiffany M W, Wang Zheng
NRL-ASEE Postdoctoral Associate in the Chemistry Division, U.S. Naval Research Laboratory, Washington, D.C. 20375, United States.
Chemistry Division, U.S. Naval Research Laboratory, Washington, D.C. 20375, United States.
ACS Omega. 2025 May 7;10(19):19808-19816. doi: 10.1021/acsomega.5c01034. eCollection 2025 May 20.
Eumelanins are a family of biomolecules with a wide range of functionality in nature and tremendous potential for commercial and industrial applications, provided that they can be produced in large quantities. Our group has developed a method to biosynthesize eumelanin with high yield using the marine bacterium Vibrio natriegens. In this work, we use high-resolution H, C, and N magic angle spinning (MAS) nuclear magnetic resonance (NMR) to characterize the purity of the extracted solid eumelanin as a function of the quality control methods. A key observation is that biomass eumelanin contains a higher level of biocontaminant compared to supernatant eumelanin. Additional insights into the efficiency of the biosynthetic pathway of eumelanin synthesis were obtained by incorporating C- and N-enriched precursors into the growth medium and subsequent MAS NMR spectra of the eumelanins. These results highlight the usefulness of solid-state NMR for assessing the quality of the eumelanins produced biosynthetically, which is critical for their further application.
真黑素是一类在自然界中具有广泛功能的生物分子，并且只要能够大量生产，就具有巨大的商业和工业应用潜力。我们的团队已经开发出一种利用海洋细菌嗜盐栖热袍菌高产生物合成真黑素的方法。在这项工作中，我们使用高分辨率的氢、碳和氮魔角旋转（MAS）核磁共振（NMR）来表征所提取的固体真黑素的纯度，该纯度是质量控制方法的函数。一个关键的观察结果是，与上清液真黑素相比，生物质真黑素含有更高水平的生物污染物。通过将富含碳和氮的前体掺入生长培养基以及随后对真黑素进行MAS NMR光谱分析，我们获得了关于真黑素生物合成途径效率的更多见解。这些结果突出了固态NMR在评估生物合成产生的真黑素质量方面的有用性，这对于它们的进一步应用至关重要。
