Department of Bariatric Surgery, The First Affiliated Hospital of Jinan University, Guangzhou, China.
Department of Pharmacy, Affiliated Cancer Hospital of Inner Mongolia Medical University, Peking University Cancer Hospital Inner Mongolia Hospital, Hohhot, China.
Front Immunol. 2024 Mar 11;15:1365457. doi: 10.3389/fimmu.2024.1365457. eCollection 2024.
Inflammatory bowel disease (IBD) greatly affects human quality of life. Mannose has been reported to be used to treat IBD, but the mechanism is currently unknown.
C57/BL mice were used as research subjects, and the mouse acute colitis model was induced using dextran sulfate sodium salt (DSS). After oral administration of mannose, the body weights and disease activity index (DAI) scores of the mice were observed. The colon lengths, histopathological sections, fecal content microbial sequencing, colon epithelial inflammatory genes, and tight junction protein Occludin-1 expression levels were measured. We further used the feces of mice that had been orally administered mannose to perform fecal bacterial transplantation on the mice with DSS-induced colitis and detected the colitis-related indicators.
Oral administration of mannose increased body weights and colon lengths and reduced DAI scores in mice with DSS-induced colitis. In addition, it reduced the expression of colon inflammatory genes and the levels of serum inflammatory factors (TNF-α, IL-6, and IL-1β), further enhancing the expression level of the colonic Occludin-1 protein and alleviating the toxic response of DSS to the intestinal epithelium of the mice. In addition, gut microbial sequencing revealed that mannose increased the abundance and diversity of intestinal flora. Additionally, after using the feces of the mannose-treated mice to perform fecal bacterial transplantation on the mice with DSS-induced colitis, they showed the same phenotype as the mannose-treated mice, and both of them alleviated the intestinal toxic reaction induced by the DSS. It also reduced the expression of intestinal inflammatory genes (TNF-α, IL-6, and IL-1β) and enhanced the expression level of the colonic Occludin-1 protein.
Mannose can treat DSS-induced colitis in mice, possibly by regulating intestinal microorganisms to enhance the intestinal immune barrier function and reduce the intestinal inflammatory response.
炎症性肠病（IBD）极大地影响了人类的生活质量。据报道，甘露糖可用于治疗 IBD，但目前其机制尚不清楚。
以 C57/BL 小鼠为研究对象，用葡聚糖硫酸钠（DSS）诱导小鼠急性结肠炎模型。甘露糖灌胃后，观察小鼠体重和疾病活动指数（DAI）评分，测量结肠长度、组织病理学切片、粪便内容物微生物测序、结肠上皮炎症基因和紧密连接蛋白 Occludin-1 表达水平。进一步将甘露糖灌胃小鼠的粪便进行 DSS 诱导结肠炎小鼠的粪便细菌移植，并检测结肠炎相关指标。
甘露糖灌胃可增加 DSS 诱导结肠炎小鼠的体重和结肠长度，降低 DAI 评分。此外，它还降低了结肠炎症基因的表达和血清炎症因子（TNF-α、IL-6 和 IL-1β）水平，进一步增强了结肠 Occludin-1 蛋白的表达水平，减轻了 DSS 对小鼠肠道上皮的毒性反应。此外，肠道微生物测序显示，甘露糖增加了肠道菌群的丰度和多样性。此外，用甘露糖处理小鼠的粪便进行 DSS 诱导结肠炎小鼠的粪便细菌移植后，它们表现出与甘露糖处理小鼠相同的表型，均减轻了 DSS 诱导的肠道毒性反应，也降低了肠道炎症基因（TNF-α、IL-6 和 IL-1β）的表达，增强了结肠 Occludin-1 蛋白的表达水平。
甘露糖可以治疗 DSS 诱导的结肠炎，可能通过调节肠道微生物来增强肠道免疫屏障功能，减轻肠道炎症反应。
