Croff J, Tan C, Chiaf A L, Hartwell M L, Crockett E K, Teague T K
Department of Rural Health, Oklahoma State University Center for Health Sciences, Tulsa, USA.
Integrative Immunology Center, University of Oklahoma-Tulsa, Tulsa, USA.
Vitam Miner. 2020;9(1). Epub 2020 Feb 10.
Public health programs aimed at identifying and monitoring individuals at risk of specific nutrient deficiencies may benefit from advances in biospecimen sampling techniques that allow for easier in-the-field collections. Such advances may be particularly important for those of childbearing potential, in order to identify individuals at risk of low folate status due to sub-optimal nutriture. Folate is a critical nutrient of interest among women of childbearing potential because suboptimal levels are a primary contributor to neural tube defects. Whatman Paper Dried Blood Spots (WDBS) are a convenient method for assessing folate; however, a major drawback of WDBS has been the inability to separate serum from erythrocyte folate in these samples.
The purpose of this study was to test the feasibility of using newer linear flow chromatography DBS cards to measure serum and erythrocyte folate.
A convenience sample (n=27) was recruited to assess folate values collected by venous blood draw, Whatman paper, and linear flow chromatography cards. These sampling techniques allowed for assessment of erythrocyte and serum folate values collected via different methods. Folate levels in the samples were assessed using standard microbiological assays.
Erythrocyte folate values from the two blood spot methodologies (Whatman paper and linear flow chromatography DBS) indicate a strong linear relationship, with 92% of variance accounted for in a linear regression analysis. Similarly, venous blood samples and linear flow chromatography DBS values accounted for 88% of the variance.
Linear flow chromatography dried blood spot cards are useful for assessment of erythrocyte and serum folate. Values had a strong, positive, linear relationship to serum and erythrocyte folate values from other validated methodologies, including Whatman dried blood spots and venous whole blood samples.
旨在识别和监测特定营养素缺乏风险个体的公共卫生项目，可能会受益于生物样本采样技术的进步，这些技术使现场采集更加便捷。对于有生育潜力的人群而言，此类进展可能尤为重要，以便识别因营养状况欠佳而存在叶酸水平偏低风险的个体。叶酸是有生育潜力女性关注的一种关键营养素，因为叶酸水平欠佳是神经管缺陷的主要成因。沃特曼滤纸干血斑（WDBS）是评估叶酸的一种便捷方法；然而，WDBS的一个主要缺点是无法在这些样本中分离血清叶酸和红细胞叶酸。
本研究的目的是测试使用新型线性流动色谱干血斑卡测量血清叶酸和红细胞叶酸的可行性。
招募了一个便利样本（n = 27），以评估通过静脉采血、沃特曼滤纸和线性流动色谱卡采集的叶酸值。这些采样技术能够评估通过不同方法采集的红细胞叶酸和血清叶酸值。样本中的叶酸水平采用标准微生物学检测方法进行评估。
两种血斑方法（沃特曼滤纸和线性流动色谱干血斑）测得的红细胞叶酸值显示出很强的线性关系，线性回归分析解释了92%的方差。同样，静脉血样本和线性流动色谱干血斑值解释了88%的方差。
线性流动色谱干血斑卡可用于评估红细胞叶酸和血清叶酸。其值与其他经过验证的方法（包括沃特曼干血斑和静脉全血样本）测得的血清叶酸和红细胞叶酸值具有很强的正线性关系。
