OsSDG715，一种组蛋白H3K9甲基转移酶，整合生长素和细胞分裂素信号以调控水稻愈伤组织的形成。
OsSDG715, a Histone H3K9 Methyltransferase, Integrates Auxin and Cytokinin Signaling to Regulate Callus Formation in Rice.
作者信息
Song Wenjing, Cai Hairong, Guo Yuanyuan, Chen Shiyi, Yao Yingyun, Wang Jiafeng, Guo Tao, Zhang Jian, Chen Chun
机构信息
National Engineering Research Center of Plant Space Breeding, South China Agricultural University, Guangzhou, 510642, China.
College of Agriculture, South China Agricultural University, Guangzhou, Guangdong, China.
出版信息
Rice (N Y). 2025 May 27;18(1):42. doi: 10.1186/s12284-025-00801-8.
Efficient callus induction is essential for the genetic transformation of rice (Oryza sativa), yet its regulatory mechanisms remain elusive. Previously, through a genome-wide association study (GWAS), we identified a significant associated locus on chromosome 8. In this study, we characterized this locus and demonstrated that OsSDG715, encoding a histone H3K9 methyltransferase, is the causal gene that positively regulates callus formation in rice. Results revealed that OsSDG715 is highly expressed during callus induction and exhibits natural variations associated with callus induction rate (CIR). Knockout of OsSDG715 via CRISPR/Cas9 led to a significant decrease in CIR and impaired callus morphology, indicating its positive regulation of callus formation. RNA-seq analyses revealed that 326 and 705 differentially expressed genes (DEGs) were upregulated and downregulated in sdg715 mutants, including auxin-responsive genes (OsIAA14, OsYUCCA6), cytokinin-related genes (OsCKX4, ARR10), and stress-responsive factors. Further analysis showed reduced endogenous indole-3-acetic acid (IAA) levels and increased zeatin levels in sdg715 mutants. These findings advance our understanding of the molecular mechanisms underlying rice callus formation, and offering valuable insights for optimizing tissue culture in molecular breeding.
高效的愈伤组织诱导对于水稻（Oryza sativa）的遗传转化至关重要，但其调控机制仍不清楚。此前，通过全基因组关联研究（GWAS），我们在8号染色体上鉴定出一个显著的关联位点。在本研究中，我们对该位点进行了表征，并证明编码组蛋白H3K9甲基转移酶的OsSDG715是正向调控水稻愈伤组织形成的因果基因。结果显示，OsSDG715在愈伤组织诱导过程中高度表达，并表现出与愈伤组织诱导率（CIR）相关的自然变异。通过CRISPR/Cas9敲除OsSDG715导致CIR显著降低，愈伤组织形态受损，表明其对愈伤组织形成的正向调控作用。RNA测序分析显示，sdg715突变体中有326个和705个差异表达基因（DEG）上调和下调，包括生长素响应基因（OsIAA14、OsYUCCA6）、细胞分裂素相关基因（OsCKX4、ARR10）和胁迫响应因子。进一步分析表明，sdg715突变体内源吲哚-3-乙酸（IAA）水平降低，玉米素水平升高。这些发现增进了我们对水稻愈伤组织形成分子机制的理解，并为优化分子育种中的组织培养提供了有价值的见解。
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