Alexander N J
Fertil Steril. 1977 May;28(5):562-9.
Rhesus monkeys develop agglutinating and complement-dependent antisperm antibodies after vasectomy. In order to study whether these antibodies affect fertility after vasovasostomy, 15 animals were given vasectomies and 6 months later vasovasostomies. Subsequently, each was mated with females of proven fertility. Five controls were given sham operations and similarly treated. During this period, each aimal was bled for serum to monitor the humoral immune response, ejaculated for semen analyses, and palpated for granuloma or fistula development. All control animals had a transient decrease in sperm density after sham vasectomy and vasovasostomy operations. The surgical procedures of vasectomy and subsequent vasovasostomy resulted in more animals having sperm of poor motility and quality. All of vasovasostomies were surgically successful in that sperm were again present in the ejaculate of each animal. The amount of sperm in the ejaculate could not be correlated with the ease of surgical procedure, presence or absence of macrophages in the ejaculum, motility, or forward progression. Only animals that had been vasectomized developed circulating antisperm antibodies. Sustained, elevated levels of antisperm antibodies most commonly occurred in monkeys that had high initial total sperm counts. Six of the experimental animals retained high levels of sperm-immobilizing antibodies after vasovasostomy. Of these, two were found to be infertile and two were classed as subfertile. Of the nine experimental animals without sustained antisperm antibody production, only one was classed as subfertile. This suggests that antisperm antibodies may in some cases impair the restoration of fertility after vasovasostomy.
恒河猴在输精管切除术后会产生凝集性和补体依赖性抗精子抗体。为了研究这些抗体是否会影响输精管吻合术后的生育能力，对15只动物进行了输精管切除术，6个月后进行了输精管吻合术。随后，让每只动物与已证实具有生育能力的雌性动物交配。5只对照动物接受了假手术并进行了类似处理。在此期间，对每只动物采血获取血清以监测体液免疫反应，采集精液进行分析，并触诊检查是否有肉芽肿或瘘管形成。所有对照动物在接受假输精管切除术和输精管吻合术后精子密度均有短暂下降。输精管切除术及随后的输精管吻合术导致更多动物的精子活力和质量较差。所有输精管吻合术在手术上都是成功的，因为每只动物的精液中再次出现了精子。精液中的精子数量与手术操作的难易程度、精液中是否存在巨噬细胞、精子活力或向前运动能力均无关联。只有接受输精管切除术的动物产生了循环抗精子抗体。抗精子抗体持续升高的水平最常出现在初始总精子数较高的猴子中。6只实验动物在输精管吻合术后仍保持高水平的精子制动抗体。其中，2只被发现不育，2只被归类为亚生育力。在9只没有持续产生抗精子抗体的实验动物中，只有1只被归类为亚生育力。这表明抗精子抗体在某些情况下可能会损害输精管吻合术后生育能力的恢复。
