Rahman Taieba Tuba, Wood Nathan, Pei Zhijian, Qin Hongmin, Mohan Padmini
Department of Industrial & Systems Engineering, Texas A&M University, College Station, TX 77843, USA.
Department of Biology, Texas A&M University, College Station, TX 77843, USA.
Biomimetics (Basel). 2025 May 8;10(5):297. doi: 10.3390/biomimetics10050297.
This study investigates how different levels of extrusion pressure during 3D printing affect the cell viability of human bronchial epithelial (HBE) cells embedded in printed samples. In this study, samples were printed at three levels of extrusion pressure. The cell viability was assessed through live/dead staining via microscopic imaging. The results show that increasing the extrusion pressure from 50 to 100 kPa led to a higher degree of cell death. These results demonstrate how the extrusion pressure affects the viability of HBE cells and provide a basis for future studies on pressure-induced responses in respiratory tissues.
本研究调查了3D打印过程中不同水平的挤出压力如何影响嵌入打印样品中的人支气管上皮（HBE）细胞的细胞活力。在本研究中，样品在三个挤出压力水平下进行打印。通过显微镜成像的活/死染色评估细胞活力。结果表明，将挤出压力从50 kPa增加到100 kPa会导致更高程度的细胞死亡。这些结果证明了挤出压力如何影响HBE细胞的活力，并为未来关于呼吸组织中压力诱导反应的研究提供了基础。
