Rahman Taieba Tuba, Wood Nathan, Akib Yeasir Mohammad, Qin Hongmin, Pei Zhijian
Department of Industrial & Systems Engineering, Texas A&M University, College Station, TX 77843, USA.
Department of Biology, Texas A&M University, College Station, TX 77843, USA.
Bioengineering (Basel). 2024 Aug 23;11(9):862. doi: 10.3390/bioengineering11090862.
This paper reports an experimental study on the compatibility of human bronchial epithelial (HBE) cells in a collagen-alginate bioink. The compatibility was assessed using the culture well method with three bioink compositions prepared from a 10% alginate solution and neutralized TeloCol-10 mg/mL collagen stock solution. Cell viability, quantified by (live cell count-dead cell count)/live cell count within the HBE cell-laden hydrogel, was evaluated using the live/dead assay method from Day 0 to Day 6. Experimental results demonstrated that the collagen-alginate 4:1 bioink composition exhibited the highest cell viability on Day 6 (85%), outperforming the collagen-alginate 1:4 bioink composition and the alginate bioink composition, which showed cell viability of 75% and 45%, respectively. Additionally, the live cell count was highest for the collagen-alginate 4:1 bioink composition on Day 0, a trend that persisted through Days 1 to 6, underscoring its superior performance in maintaining cell viability and promoting cell proliferation. These findings show that the compatibility of HBE cells with the collagen-alginate 4:1 bioink composition was higher compared with the other two bioink compositions.
本文报道了一项关于人支气管上皮（HBE）细胞在胶原-海藻酸盐生物墨水体系中相容性的实验研究。采用培养孔法评估相容性，所用的三种生物墨水组合物由10%海藻酸盐溶液和中和后的TeloCol-10 mg/mL胶原储备溶液制备而成。通过活/死细胞检测法，在第0天至第6天评估负载HBE细胞的水凝胶中的细胞活力，细胞活力通过（活细胞数-死细胞数）/活细胞数进行量化。实验结果表明，胶原-海藻酸盐4:1生物墨水组合物在第6天表现出最高的细胞活力（85%），优于胶原-海藻酸盐1:4生物墨水组合物和海藻酸盐生物墨水组合物，后两者的细胞活力分别为75%和45%。此外，胶原-海藻酸盐4:1生物墨水组合物在第0天的活细胞数最高，这一趋势在第1天至第6天持续存在，突出了其在维持细胞活力和促进细胞增殖方面的优越性能。这些研究结果表明，与其他两种生物墨水组合物相比，HBE细胞与胶原-海藻酸盐4:1生物墨水组合物的相容性更高。
