Rahman Taieba Tuba, Wood Nathan, Pei Zhijian, Qin Hongmin
Department of Industrial & Systems Engineering, Texas A&M University, College Station, TX 77843, USA.
Department of Biology, Texas A&M University, College Station, TX 77843, USA.
Bioengineering (Basel). 2024 Nov 28;11(12):1201. doi: 10.3390/bioengineering11121201.
This paper reports an experimental study on the effects of extreme temperature on human bronchial epithelial (HBE) cells encapsulated in 3D printed samples. Well plates of the 3D printed samples were exposed to three levels of temperature (37 °C, 45 °C, and 55 °C, respectively) for a duration of 10 min. Cells' responses, specifically cell viability and oxidative stress, were quantified using Hoechst 33342, Sytox, and Mitosox stains, with intensity measurements obtained via a plate reader. In addition, cell viability was assessed through microscopic imaging of the 3D printed samples. Experimental results demonstrated that the temperature increase from 37 °C to 55 °C significantly reduced nuclear integrity as observed through Hoechst 33342 intensity, while increased Sytox intensity reflected a higher degree of cell death. Furthermore, cells exposed to 45 °C and 55 °C exhibited decreased cell viability and elevated mitochondrial oxidative stress. These findings offer valuable insights into the effects of extreme temperature on HBE cells, establishing a foundation for future research into how respiratory tissues respond to thermal stress. This research can potentially advance the knowledge regarding effects of heat exposure on the respiratory system.
本文报道了一项关于极端温度对封装在3D打印样品中的人支气管上皮（HBE）细胞影响的实验研究。将3D打印样品的孔板分别暴露于三种温度水平（37°C、45°C和55°C）下10分钟。使用Hoechst 33342、Sytox和Mitosox染色剂对细胞反应，特别是细胞活力和氧化应激进行定量，并通过酶标仪获得强度测量值。此外，通过对3D打印样品进行显微镜成像来评估细胞活力。实验结果表明，从37°C升高到55°C显著降低了通过Hoechst 33342强度观察到的核完整性，而Sytox强度增加反映了更高程度的细胞死亡。此外，暴露于45°C和55°C的细胞表现出细胞活力下降和线粒体氧化应激升高。这些发现为极端温度对HBE细胞的影响提供了有价值的见解，为未来研究呼吸组织如何应对热应激奠定了基础。这项研究有可能推进关于热暴露对呼吸系统影响的知识。
