Bai Ruxue, Wang Qingchun, Bao Haiying
Key Laboratory for Development and Utilization of Fungi Traditional Chinese Medicine Resources, Jilin Agricultural University, Changchun 130118, China.
Key Laboratory of Edible Fungal Resources and Utilization (North), Ministry of Agriculture and Rural Affairs, Jilin Agricultural University, Changchun 130118, China.
J Fungi (Basel). 2025 Apr 29;11(5):346. doi: 10.3390/jof11050346.
growing on is traditionally regarded as the authentic source of the medicinal fungus. However, this species is also found on other host trees, such as and ; yet, whether these strains share comparable genomic and functional traits with -derived strains remains unknown. Here, we performed whole-genome sequencing of a strain isolated from (UMI) using Illumina and PacBio platforms and conducted comparative genomic analysis with strains from (MAI) and (AMI). Antagonistic interactions were also evaluated via dual-culture confrontation assays. The UMI genome was 36.44 Mb in size, comprising 9097 predicted genes, of which 6991 and 1672 were annotated in the KEGG and COG databases, respectively. SNP analysis revealed 623,498 and 335,343 variants in AMI and MAI, with AMI showing greater genomic variation. Core-pan genome analysis identified 2651 core genes and 1046, 1424, and 1217 strain-specific genes in UMI, AMI, and MAI, respectively. Phenotypic assays demonstrated distinct mycelial growth dynamics and antagonistic behaviors, which likely reflect host-related environmental adaptation. Overall, strains from non- hosts exhibit unique genomic and phenotypic features, providing a valuable basis for resource evaluation, artificial domestication, and the medicinal development of wild Sanghuang strains beyond traditional sources.
传统上认为生长在[具体树种1]上的是这种药用真菌的正宗来源。然而，在其他寄主树上也发现了该物种，比如[具体树种2]和[具体树种3]；不过，这些菌株与源自[具体树种1]的菌株是否具有可比的基因组和功能特征仍不清楚。在此，我们使用Illumina和PacBio平台对从[具体树种2]分离的一株菌株（UMI）进行了全基因组测序，并与源自[具体树种1]（MAI）和[具体树种3]（AMI）的菌株进行了比较基因组分析。还通过双培养对峙试验评估了拮抗相互作用。UMI基因组大小为36.44 Mb，包含9097个预测基因，其中分别有6991个和1672个在KEGG和COG数据库中得到注释。SNP分析在AMI和MAI中分别揭示了623,498个和335,343个变异，AMI显示出更大的基因组变异。核心-泛基因组分析在UMI、AMI和MAI中分别鉴定出2651个核心基因以及1046个、1424个和1217个菌株特异性基因。表型试验表明了不同的菌丝生长动态和拮抗行为，这可能反映了与寄主相关的环境适应性。总体而言，来自非传统寄主的菌株表现出独特的基因组和表型特征，为资源评估、人工驯化以及传统来源之外野生桑黄菌株的药用开发提供了有价值的基础。
