Wu Tuheng, Cai Manjun, Hu Huiping, Jiao Chunwei, Zhang Zhi, Liu Yuanchao, Chen Jian, Xiao Chun, Li Xiangmin, Gao Xiong, Chen Shaodan, Wu Qingping, Xie Yizhen
Guangdong Yuewei Edible Fungi Technology Co., Guangzhou 510000, China.
School of Bioscience and Bioengineering, South China University of Technology, Guangzhou 510000, China.
J Fungi (Basel). 2022 Oct 14;8(10):1081. doi: 10.3390/jof8101081.
is an important medicinal mushroom widely cultured in Asian countries. Exopolysaccharides are bioactive compounds of with health benefits. Limited exopolysaccharide content hinders its extraction from . The addition of Tween80 had an enhanced effect on exopolysaccharide production in submerged fermentation. However, the mechanism of this effect remains unclear. In this study, we report on a high-quality assembly of strain yw-1-5 to lay the foundation for further transcriptome analysis. The genome sequence was 58.16 Mb and consisted of 58 scaffolds with an N50 of 4.78 Mb. A total of 13,957 protein-coding genes were annotated and Hi-C data mapped to 12 pseudo-chromosomes. Genes encoding glycosyltransferases and glycoside hydrolases were also obtained. Furthermore, RNA-seq was performed in a Tween80-treated group and control group for revealing the enhanced effect of Tween80 on exopolysaccharide production. In total, 655 genes were identified as differentially expressed, including 341 up-regulated and 314 down-regulated. Further analysis of differentially expressed genes showed that groups of MAPK, amino sugar and nucleotide sugar metabolism, autophagy, ubiquitin-mediated proteolysis, peroxisome, starch and sucrose metabolism, TCA cycle, glycolysis/gluconeogenesis KEGG pathway, glycosyltransferases and glycoside hydrolases played important roles in the enhanced effect of Tween80 on exopolysaccharide production. This work provides a valuable resource for facilitating our understanding of the synthesis of polysaccharides and accelerating the breeding of new strains with a high content of exopolysaccharides.
是一种在亚洲国家广泛栽培的重要药用蘑菇。胞外多糖是具有健康益处的生物活性化合物。有限的胞外多糖含量阻碍了从其提取。吐温80的添加对其在深层发酵中产生胞外多糖有增强作用。然而，这种作用的机制仍不清楚。在本研究中，我们报道了菌株yw-1-5的高质量组装，为进一步的转录组分析奠定基础。基因组序列为58.16 Mb，由58个支架组成，N50为4.78 Mb。总共注释了13957个蛋白质编码基因，并将Hi-C数据映射到12条假染色体上。还获得了编码糖基转移酶和糖苷水解酶的基因。此外，在吐温80处理组和对照组中进行了RNA测序，以揭示吐温80对胞外多糖产生的增强作用。总共鉴定出655个差异表达基因，包括341个上调基因和314个下调基因。对差异表达基因的进一步分析表明，丝裂原活化蛋白激酶、氨基糖和核苷酸糖代谢、自噬、泛素介导的蛋白水解、过氧化物酶体、淀粉和蔗糖代谢、三羧酸循环（TCA循环）、糖酵解/糖异生KEGG途径、糖基转移酶和糖苷水解酶在吐温80对胞外多糖产生的增强作用中起重要作用。这项工作为促进我们对多糖合成的理解和加速高含量胞外多糖新菌株的育种提供了宝贵资源。
