Zhang Xinyu, Zu Qianhe, Deng Chunlin, Gao Xin, Liu Hongxu, Jin Yi, Yang Xinjian, Wang Enjun
College of Nursing, Hebei University, Baoding 071002, China.
College of Basic Medical Science, Key Laboratory of Pathogenesis Mechanism and Control of Inflammatory-Autoimmune Diseases of Hebei Province, Hebei University, Baoding 071002, China.
J Funct Biomater. 2025 May 7;16(5):164. doi: 10.3390/jfb16050164.
Scarring is a prevalent and often undesirable outcome of the wound healing process, impacting millions worldwide. The complex and dynamic nature of wound healing, including hemostasis, inflammation, proliferation, and remodeling, necessitates precise, making it hard for stage-specific interventions to prevent pathological scarring. This study introduces a double-layer hydrogel system designed for sequential drug release, aligning with the stage-specific need for wound healing. The lower layer, containing curcumin-loaded chitosan nanoparticles, shows early anti-inflammatory and antioxidant effects, while the upper layer, with pirfenidone-encapsulated gelatin microspheres, presents late-stage anti-fibrotic activity. The hydrogel's unique design, with varying degradation rates and mechanical properties in each layer, facilitates cascade drug release in synchrony with wound healing stages. Rapid release of curcumin from the lower layer promotes proliferation by mitigating inflammation and oxidative stress, while the sustained release of pirfenidone from the upper layer inhibits excessive fibrillation during late proliferation and remodeling. In a rat model of full-thickness skin defect, treatment with a double-layer hydrogel drug delivery system accelerated the wound closure, improved scar quality, and promoted the formation of hair follicles. Therefore, this innovative approach lays a promising foundation for future clinical applications in anti-scar therapies, offering a significant advancement in wound care and regenerative medicine.
瘢痕形成是伤口愈合过程中普遍存在且往往不受欢迎的结果，影响着全球数百万人。伤口愈合的复杂动态性质，包括止血、炎症、增殖和重塑，需要精确控制，这使得针对特定阶段的干预措施难以预防病理性瘢痕形成。本研究介绍了一种设计用于顺序药物释放的双层水凝胶系统，符合伤口愈合的特定阶段需求。下层含有负载姜黄素的壳聚糖纳米颗粒，具有早期抗炎和抗氧化作用，而上层含有包裹吡非尼酮的明胶微球，具有后期抗纤维化活性。水凝胶的独特设计，每层具有不同的降解速率和机械性能，有助于与伤口愈合阶段同步进行级联药物释放。姜黄素从下层快速释放，通过减轻炎症和氧化应激促进增殖，而吡非尼酮从上层持续释放，在后期增殖和重塑过程中抑制过度纤维化。在全层皮肤缺损的大鼠模型中，双层水凝胶药物递送系统治疗加速了伤口闭合，改善了瘢痕质量，并促进了毛囊形成。因此，这种创新方法为未来抗瘢痕治疗的临床应用奠定了有前景的基础，在伤口护理和再生医学方面取得了重大进展。
