Hu Yulong, Wu Yihan, Wang Chunlong, Jin Qiguan, Chen Xianghe
College of Physical Education, Yangzhou University, Yangzhou 225009, China.
Metabolites. 2025 May 16;15(5):331. doi: 10.3390/metabo15050331.
Obesity, marked by excessive white adipose tissue (WAT) accumulation, worsens metabolic disorders, and inducing WAT browning is a promising therapy. This study examined the synergistic effects of moderate-intensity aerobic training and resveratrol (RES) on WAT browning and its underlying mechanisms in obese male rats. Male Sprague Dawley rats were divided into a normal diet control group ( = 8) and a high-fat-diet modeling group ( = 32), with the rats in the latter group being further divided randomly in groups of eight into a high-fat group; a high-fat, exercise group; a high-fat, RES group; and a high-fat, exercise-combined-with-RES group. The rats in the exercise intervention groups underwent moderate-intensity aerobic treadmill exercise for one hour daily, six days a week, while those in the RES groups received a 50 mg/kg/d RES solution via gavage before exercise, once daily, six days a week. Both interventions lasted eight weeks. The combined intervention synergistically suppressed weight gain and visceral fat accumulation. WAT browning was enhanced, evidenced by upregulated UCP1 and CIDEA expression. Mitochondrial biogenesis was activated via the SIRT1-PGC-1α-NRF-1-TFAM pathway, accompanied by elevated mitochondrial enzyme activity and improved lipid mobilization (reduced serum free fatty acids and triglycerides). The combination of aerobic exercise and RES promotes WAT browning and lipolysis by enhancing mitochondrial biogenesis and stimulating mitochondrial thermogenesis through the modulation of the SIRT1-PGC-1α-NRF-1-TFAM pathway.
肥胖以白色脂肪组织（WAT）过度堆积为特征，会加重代谢紊乱，而诱导WAT褐变是一种很有前景的治疗方法。本研究考察了中等强度有氧运动和白藜芦醇（RES）对肥胖雄性大鼠WAT褐变的协同作用及其潜在机制。将雄性Sprague Dawley大鼠分为正常饮食对照组（n = 8）和高脂饮食建模组（n = 32），后者再随机分为高脂组、高脂运动组、高脂RES组和高脂运动联合RES组，每组8只。运动干预组的大鼠每天进行1小时中等强度的有氧跑步机运动，每周6天；RES组的大鼠在运动前每天经口灌胃给予50 mg/kg/d的RES溶液，每周6天。两种干预均持续8周。联合干预协同抑制了体重增加和内脏脂肪堆积。UCP1和CIDEA表达上调证明WAT褐变增强。通过SIRT1-PGC-1α-NRF-1-TFAM途径激活了线粒体生物发生，同时线粒体酶活性升高，脂质动员改善（血清游离脂肪酸和甘油三酯降低）。有氧运动和RES的联合通过增强线粒体生物发生以及通过调节SIRT1-PGC-1α-NRF-1-TFAM途径刺激线粒体产热，促进WAT褐变和脂肪分解。
