Vadankula Govinda Raju, Rizvi Arshad, Ali Haider, Khunjamayum Rakhi, Eedara V V Ramprasad, Nema Vijay, Ningthoujam Debananda Singh, Suresh Babu Katragadda, Shetty Prakasham Reddy, Mande Shekhar C, Banerjee Sharmistha
Laboratory of Molecular Pathogenesis, Department of Biochemistry, School of Life Sciences, University of Hyderabad (UoH), Hyderabad 500046, India.
Microbial Biotechnology Research Laboratory (MBRL), Department of Biochemistry, Manipur University, Canchipur 795003, India.
Trop Med Infect Dis. 2025 Apr 23;10(5):117. doi: 10.3390/tropicalmed10050117.
The long regimen of drug therapy, the emergence of drug-resistance (DR), and infections with non-tuberculous mycobacteria (NTMs) are alarming challenges in controlling tuberculosis (TB), a disease caused by (), necessitating the pursuit of new, broad-spectrum anti-mycobacterials. With more than two-thirds of the clinically useful antibiotics originating from the bacterial phylum Actinomycetota, and their enormous diversity in India, we explored atypical environments for new bacterial strains with potential anti- activity. In this study, we the examined the secondary metabolites of soil and endophytic bacterial isolates from the wetland niches of Manipur, India, and determined their anti-mycobacterial properties using viability assays. The ethyl acetate culture filtrate extracts of one of the isolates, named sp. SbAr007, showed broad-spectrum anti-mycobacterial activity against laboratory strains H37Ra and H37Rv, a clinical drug-resistant and non-tuberculous mycobacteria (NTM). The isolate was characterized for its phenotype and genetic identity, which indicated its closeness to , , and . Further, macrophage infection assays showed that the extracts could effectively control the intracellular mycobacterial growth but had negligible cytotoxicity to PBMCs from healthy donors. LC-MS identified an unusual combination of antibiotics in these culture filtrate extracts, which can be further explored for specific active molecules or as a formulation against DR-TB.
长期的药物治疗方案、耐药性的出现以及非结核分枝杆菌感染，是控制结核病（一种由……引起的疾病）过程中令人担忧的挑战，这使得人们有必要寻找新型的广谱抗分枝杆菌药物。由于超过三分之二的临床可用抗生素源自放线菌门细菌，且印度的这类细菌具有巨大的多样性，我们探索了非典型环境以寻找具有潜在抗……活性的新菌株。在本研究中，我们检测了来自印度曼尼普尔邦湿地生态位的土壤和内生细菌分离株的次生代谢产物，并通过活力测定法确定了它们的抗分枝杆菌特性。其中一种名为sp. SbAr007的分离株的乙酸乙酯培养滤液提取物，对实验室菌株H37Ra和H37Rv、一种临床耐药……以及非结核分枝杆菌表现出广谱抗分枝杆菌活性。对该分离株进行了表型和基因鉴定，结果表明它与……、……和……关系密切。此外，巨噬细胞感染试验表明，这些提取物可以有效控制细胞内分枝杆菌的生长，但对健康供体的外周血单核细胞的细胞毒性可忽略不计。液相色谱 - 质谱联用技术鉴定出这些培养滤液提取物中存在不同寻常的抗生素组合，可进一步探索其中的特定活性分子或作为抗耐药结核病的制剂。
