Wang Hongsen, Xing Shu, Chen Chonghao, Si Zhangyong, Li Meng, Wang Bing, Zhou Feng, Zhang Jiantao
Laboratory of Advanced Theranostic Materials and Technology, Ningbo Institute of Materials Technology and Engineering, Chinese Academy of Sciences, Ningbo 315201, PR China.
Ningbo Cixi institute of Biomedical Engineering, Cixi, 315300, PR China.
J Mater Chem B. 2025 Jun 25;13(25):7311-7319. doi: 10.1039/d5tb00391a.
As a novel approach to killing bacteria, photodynamic therapy holds great potential in antibacterial treatment. However, the majority of traditional photosensitizers exhibit relatively low reactive oxygen species (ROS) quantum yield. Therefore, it is essential to develop photosensitizers with high ROS quantum yield to effectively kill bacteria. Herein, we propose a molecular design approach to enhance the spin-orbit coupling (SOC) and improve the ROS quantum yield by introducing carbonyl groups into a donor-acceptor (D-A) system. In the meantime, we also introduced membrane-anchoring functional groups to the photosensitizer to anchor on the bacterial surface for improved antibacterial treatment. In this design, two D-A photosensitizers (CTI-1-anchor and CTI-2-anchor) were synthesized by linking membrane-anchoring functional groups to carbazole and indanedione derivatives. Notably, the resulting CTI-1-anchor exhibited a significantly enhanced ROS generation capability, and its ROS quantum yield can reach 87%. Moreover, the CTI-1-anchor demonstrated superior antibacterial performance against Gram-positive bacteria () and Gram-negative bacteria (). The antibacterial efficacy of CTI-1-anchor reached 97.7% and 73.4% for and , respectively. This study is expected to inspire further molecular designs of photosensitizers, ultimately contributing to the development of efficient antibacterial therapy.
作为一种新型的杀菌方法，光动力疗法在抗菌治疗中具有巨大潜力。然而，大多数传统光敏剂的活性氧（ROS）量子产率相对较低。因此，开发具有高ROS量子产率的光敏剂以有效杀灭细菌至关重要。在此，我们提出一种分子设计方法，通过在供体-受体（D-A）体系中引入羰基来增强自旋-轨道耦合（SOC）并提高ROS量子产率。同时，我们还在光敏剂中引入膜锚定官能团，使其锚定在细菌表面以改善抗菌治疗效果。在该设计中，通过将膜锚定官能团连接到咔唑和茚二酮衍生物上，合成了两种D-A光敏剂（CTI-1-anchor和CTI-2-anchor）。值得注意的是，所得的CTI-1-anchor表现出显著增强的ROS生成能力，其ROS量子产率可达87%。此外，CTI-1-anchor对革兰氏阳性菌（）和革兰氏阴性菌（）均表现出优异的抗菌性能。CTI-1-anchor对 和 的抗菌效率分别达到97.7%和73.4%。本研究有望激发对光敏剂的进一步分子设计，最终推动高效抗菌疗法的发展。
