Dias Paulo Jorge
iBB-Institute for Bioengineering and Biosciences, Instituto Superior Técnico, University of Lisbon, 1049-001 Lisboa, Portugal.
Associate Laboratory i4HB-Institute for Health and Bioeconomy at Instituto Superior Técnico, University of Lisbon, 1049-001 Lisboa, Portugal.
FEMS Yeast Res. 2025 Jan 30;25. doi: 10.1093/femsyr/foaf026.
The transporters of the ATP-Binding Cassette (ABC) Superfamily involved in the Multidrug Resistance (MDR) phenomena are also known as ABC-Pleiotropic Drug Resistance (PDR) proteins. The homologs of the Saccharomyces cerevisiae SNQ2 and PDR18 genes were identified in 171 yeast genomes, representing 68 different hemiascomycetous species. All early-divergent yeast species analyzed in this work lack Snq2/Pdr18 homologs, suggesting that the origin of these ABC-PDR genes in hemiascomycete yeasts resulted from a horizontal transfer event. The evolutionary pathway of the Snq2/Pdr18 protein subfamily in pathogenic Candida species was also reconstructed, revealing a main gene lineage leading to the Candida albicans SNQ2 gene. The results indicate that, after the gene duplication event at the origin of the SNQ2/PDR18 paralogs, the PDR18 ortholog has been under strong diversifying selection and suggest that a small portion of the sequence of the SNQ2 ancestral ortholog might have been under mild positive selection. The results also showed that strong positive selection was exerted over one of the two paralogs generated by the Whole Genome Duplication (WGD) event, corresponding to the duplicate at the origin of a "short-lived" WGD sublineage.
参与多药耐药（MDR）现象的ATP结合盒（ABC）超家族转运蛋白也被称为ABC-多效耐药（PDR）蛋白。在171个酵母基因组中鉴定出酿酒酵母SNQ2和PDR18基因的同源物，这些基因组代表68个不同的半子囊菌物种。在这项研究中分析的所有早期分化酵母物种都缺乏Snq2/Pdr18同源物，这表明半子囊菌酵母中这些ABC-PDR基因的起源是由水平转移事件导致的。还重建了致病性念珠菌属物种中Snq2/Pdr18蛋白亚家族的进化途径，揭示了一条导致白色念珠菌SNQ2基因的主要基因谱系。结果表明，在SNQ2/PDR18旁系同源物起源处的基因复制事件后，PDR18直系同源物一直处于强烈的多样化选择之下，并表明SNQ2祖先直系同源物的一小部分序列可能处于温和的正选择之下。结果还表明，在全基因组复制（WGD）事件产生的两个旁系同源物之一上施加了强烈的正选择，该旁系同源物对应于一个“短命”WGD亚谱系起源处的重复基因。
