Mutua Maurine Mumo, Rimal Sandesh, Shrestha Sabin, Kharbuja Anjana, Dulal Anjali, Subedi Suprabha, Bhandari Dumre Renu, Khadka Ashma, Gautam Ishan, Luitel Himal, Kapandji Merveille, Inoue Shingo, Takamatsu Yuki, Pandey Basu Dev, Kaneko Satoshi, Urano Takeshi, Morita Kouichi, Dumre Shyam Prakash, Ngwe Tun Mya Myat
Department of Virology, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan.
Graduate School of Health, Kenya Medical Research Institute, Nairobi, Kenya.
Am J Trop Med Hyg. 2025 May 27;113(2):378-381. doi: 10.4269/ajtmh.24-0685. Print 2025 Aug 6.
The dengue virus (DENV) and chikungunya virus (CHIKV) infection present with similar symptoms and are transmitted by Aedes mosquitoes. Until now, there have been no reported cases of DENV and CHIKV being detected in mosquitoes in Nepal. This study identified circulating arboviruses from mosquitoes collected in the capital city Kathmandu, and Dhading, Bhaktapur, and Lalitpur districts of Central Nepal in 2023. Of 105 Aedes pooled samples, 16 (15.2%) were positive for DENV, and six (5.7%) were positive for CHIKV by quantitative real-time polymerase chain reaction. DENV-2 was the most common serotype (11.4%), aligning with recent outbreaks in Nepal in 2023, followed by DENV-3 and DENV-4 (1.9% each). To the best of our knowledge, our study is the first report to detect DENV and CHIKV in Aedes mosquitoes circulating in Nepal. This study highlights the need for mosquito surveillance to prevent future outbreaks of mosquito-borne diseases in Nepal.
登革热病毒（DENV）和基孔肯雅病毒（CHIKV）感染症状相似，均由伊蚊传播。截至目前，尼泊尔尚无在蚊子中检测到登革热病毒和基孔肯雅病毒的报告病例。本研究对2023年在尼泊尔首都加德满都以及尼泊尔中部的达丁、巴克塔普尔和拉利特布尔地区采集的蚊子中传播的虫媒病毒进行了鉴定。在105份伊蚊混合样本中，通过定量实时聚合酶链反应检测发现，16份（15.2%）登革热病毒呈阳性，6份（5.7%）基孔肯雅病毒呈阳性。登革热病毒2型是最常见的血清型（11.4%），这与2023年尼泊尔近期的疫情相符，其次是登革热病毒3型和4型（各占1.9%）。据我们所知，本研究是首次报告在尼泊尔传播的伊蚊中检测到登革热病毒和基孔肯雅病毒。这项研究凸显了开展蚊虫监测以预防尼泊尔未来蚊媒疾病爆发的必要性。
