尼日利亚驴体内[具体病原体未给出]的流行率、抗菌药物耐药性及毒力潜力

Prevalence, Antimicrobial Resistance, and Virulence Potential of in Donkeys from Nigeria.

作者信息

Okorie-Kanu Onyinye Josephine, Anyanwu Madubuike Umunna, Nwobi Obichukwu Chisom, Tambe-Ebot Regina Yaya, Ikenna-Ezeh Nkechi Harriet, Okolo Chukwuemeka Calistus, Obodoechi Lynda Onyinyechi, Ugwu Patience Chinasa, Okosi Ifeyinwa Riona, Jaja Ishmael Festus, Oguttu James Wabwire

机构信息

Department of Veterinary Public Health and Preventive Medicine, University of Nigeria, Nsukka 402001, Nigeria.

Department of Veterinary Microbiology and Immunology, University of Nigeria, Nsukka 402001, Nigeria.

出版信息

Antibiotics (Basel). 2025 Apr 29;14(5):453. doi: 10.3390/antibiotics14050453.

DOI:10.3390/antibiotics14050453

PMID:40426520

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12108190/

Abstract

BACKGROUND

Animal-associated antimicrobial-resistant staphylococci pose a One Health concern, as they can spread into the environment and cause serious infections. Yet, donkeys in Nigeria have been largely overlooked as potential reservoirs of these pathogens.

AIM/OBJECTIVES: To isolate from donkeys in Obollo-Afor, southeast Nigeria, assess their antimicrobial resistance profiles, and evaluate their virulence potential.

MATERIALS AND METHODS

Staphylococci were isolated from the nasal swabs of 250 donkeys, using mannitol salt agar, confirmed biochemically, with identified via a latex agglutination test and mass spectrometry. The resistance profiles of the isolates, including in regard to methicillin, inducible clindamycin, and β-lactamase production, were determined using disc diffusion, while vancomycin resistance was assessed through the use of agar dilution. The virulence factors were evaluated phenotypically.

RESULTS

Of the 250 samples, 11 (4.4%) contained . and 239 (95.6%) grew other species. The resistance rates of the 11 . isolates to gentamicin, penicillin, tigecycline, cefoxitin, linezolid, and chloramphenicol were 45.5%, 66.7%, 54.5%, 27.3%, 36.4%, and 18.1%, respectively. The phenotypic methicillin-resistant . prevalence was 1.2%. Additionally, 23.5% of the . isolates were multidrug resistant, with a mean antibiotic resistance index of 0.25. All the . isolates exhibited virulence factors like clumping factor expression, catalase, caseinase, lecithinase, and gelatinase activity, while the occurrence of haemagglutinin, biofilm, pellicle, and hemolysin occurred in 27.3%, 54.5%, 36.4%, 72.2%, respectively.

CONCLUSION

Although a small percentage of donkeys in Nigeria may harbor . , these animals are potentially spreading antimicrobial resistance, including multidrug and methicillin resistance, to humans and the environment.

摘要

背景

与动物相关的耐抗菌药葡萄球菌引发了“同一健康”问题，因为它们可传播到环境中并导致严重感染。然而，尼日利亚的驴子在很大程度上被忽视了，未被视为这些病原体的潜在宿主。

目的

从尼日利亚东南部奥博洛-阿福尔的驴子中分离葡萄球菌，评估它们的抗菌药耐药谱，并评估它们的毒力潜力。

材料与方法

使用甘露醇盐琼脂从250头驴子的鼻拭子中分离葡萄球菌，通过生化方法进行确认，通过乳胶凝集试验和质谱法鉴定菌株。使用纸片扩散法测定分离株的耐药谱，包括对甲氧西林、诱导型克林霉素和β-内酰胺酶产生的耐药情况，而通过琼脂稀释法评估万古霉素耐药性。对毒力因子进行表型评估。

结果

在250个样本中，11个（4.4%）含有金黄色葡萄球菌。239个（95.6%）培养出其他葡萄球菌属物种。11株金黄色葡萄球菌对庆大霉素、青霉素、替加环素、头孢西丁、利奈唑胺和氯霉素的耐药率分别为45.5%、66.7%、54.5%、27.3%、36.4%和18.1%。表型耐甲氧西林金黄色葡萄球菌的患病率为1.2%。此外，23.5%的金黄色葡萄球菌分离株对多种药物耐药，平均抗生素耐药指数为0.25。所有金黄色葡萄球菌分离株均表现出如聚集因子表达、过氧化氢酶、酪蛋白酶、卵磷脂酶和明胶酶活性等毒力因子，而血凝素、生物膜、菌膜和溶血素的发生率分别为27.3%、54.5%、36.4%、72.2%。