Wang Liyuan, Li Tong, Ma Qiugang, Qu Honglei, Wang Changfa, Liu Wenqiang, Chai Wenqiong
Liaocheng Research Institute of Donkey High-Efficiency Breeding and Ecological Feeding, Liaocheng University, Liaocheng 252000, China.
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China.
Animals (Basel). 2025 May 15;15(10):1435. doi: 10.3390/ani15101435.
The growth traits of donkeys from the same farm under the same feeding conditions often vary.
In this study, Plasma hormone level and LC-MS-based metabolomics was used to identify the metabolic pathways and the key metabolites associated with the growth rate of Dezhou donkeys.
The level of IGF-1 in the SG was significantly higher than that in the FG. The differentially abundant metabolites were related mainly to lipid metabolism, in which arachidonic acid metabolism, linoleic acid metabolism and steroid hormone biosynthesis played key roles. The main differentially abundant metabolites 2,3-dinor-8-iso-PGF2α, 11-DH-TXB2, 8(R)-HPETE, PGJ2, c9, t11-CLA, 12,13-DHOME, 9,10-DHOME, 9(10)-EpOME, 13-HPODE, DHEAS, testosterone, and corticosterone played important roles in metabolic homeostasis and affected the adaptation of donkeys to cold environments.
The present study revealed that the growth rate of donkeys is mainly influenced by their adaptation to the environment, providing a more in-depth study on the relationship between plasma metabolomics and growth rate in donkeys.
在相同饲养条件下，来自同一养殖场的驴的生长性状往往存在差异。
本研究采用血浆激素水平检测和基于液相色谱-质谱联用的代谢组学方法，以鉴定与德州驴生长速率相关的代谢途径和关键代谢物。
SG组中IGF-1水平显著高于FG组。差异丰富的代谢物主要与脂质代谢有关，其中花生四烯酸代谢、亚油酸代谢和类固醇激素生物合成起关键作用。主要差异丰富的代谢物2,3-二去甲-8-异前列腺素F2α、11-脱氢血栓素B2、8(R)-氢过氧化二十碳四烯酸、前列腺素J2、顺-9，反-11-共轭亚油酸、12,13-二羟基十八碳烯酸、9,10-二羟基十八碳烯酸、9(10)-环氧十八碳烯酸、13-氢过氧化十八碳烯酸、硫酸脱氢表雄酮、睾酮和皮质酮在代谢稳态中起重要作用，并影响驴对寒冷环境的适应。
本研究揭示了驴的生长速率主要受其对环境的适应性影响，为深入研究驴血浆代谢组学与生长速率之间的关系提供了依据。
