Zannotti Michele, Battelli Marco, Parma Pietro
Department of Agricultural and Environmental Sciences, University of Milan, Via Celoria 2, 20133 Milano, Italy.
Animals (Basel). 2025 May 20;15(10):1476. doi: 10.3390/ani15101476.
In cytogenetics, the ability to perform FISH (Fluorescence In Situ Hybridization) experiments using probes that map very closely together depends on the capacity to produce sufficiently long chromosomes. Traditionally, colcemid is the chemical agent used to obtain metaphase spreads. However, various substances have been reported to arrest cells in an earlier stage of mitosis than the metaphase, potentially providing longer chromosomes. In this study, we tested seven substances different from colcemid, which, according to the literature, have this capability: Vinblastine, Combretastatin A-4, Podophyllotoxin, Org9935, Nocodazole, Paclitaxel, and Griseofulvin. All substances were tested on lymphocyte cultures derived from whole blood at the same concentration: 0.1 µg/mL. Among these, Org9935 and Griseofulvin were confirmed to have the ability to produce metaphases with longer chromosomes compared to those obtained with colcemid.
在细胞遗传学中，使用紧密连锁的探针进行荧光原位杂交（FISH）实验的能力取决于产生足够长染色体的能力。传统上，秋水仙酰胺是用于获得中期染色体铺展的化学试剂。然而，据报道，各种物质可使细胞在有丝分裂的比中期更早阶段停滞，这可能会产生更长的染色体。在本研究中，我们测试了七种不同于秋水仙酰胺的物质，根据文献，它们具有这种能力：长春碱、康普瑞他汀A - 4、鬼臼毒素、Org9935、诺考达唑、紫杉醇和灰黄霉素。所有物质均以相同浓度0.1μg/mL在源自全血的淋巴细胞培养物上进行测试。其中，与使用秋水仙酰胺获得的中期染色体相比，Org9935和灰黄霉素被证实有能力产生更长染色体的中期染色体。
