Abramova E, Vyguinnyi S, Mamaeva S
Institute of Cytology, Academy of Sciences, St. Petersburg, Russia.
Cancer Genet Cytogenet. 1993 Jul 1;68(1):52-9. doi: 10.1016/0165-4608(93)90074-v.
The effect of Colcemid on satellite association frequencies was investigated in human cell lines U-937 GTB, SC-N-MC, HL-60, and Raji by silver staining method. Cell cultures were exposed to Colcemid at a concentration of 0.02 mg/ml for 15-60 minutes. As exposure time to Colcemid was increased, both the frequency of cells with satellite associations and the number of chromosomes involved in satellite associations increased significantly. Furthermore, the mean number of silver-stained chromosomes decreased with longer exposure time. Without Colcemid, we were able to obtain an adequate number of good-quality metaphase spreads. The data obtained may be of help in furthering research concerning satellite associations and in obtaining better quality chromosome preparations.
通过银染法研究了秋水仙酰胺对人细胞系U-937 GTB、SC-N-MC、HL-60和Raji中随体联合频率的影响。将细胞培养物暴露于浓度为0.02 mg/ml的秋水仙酰胺中15至60分钟。随着秋水仙酰胺暴露时间的增加，具有随体联合的细胞频率以及参与随体联合的染色体数量均显著增加。此外，银染染色体的平均数量随暴露时间延长而减少。在不使用秋水仙酰胺的情况下，我们能够获得足够数量的高质量中期分裂相。所获得的数据可能有助于推进有关随体联合的研究，并有助于获得质量更好的染色体标本。
