Gao Peng, Nie Yuanyang, Zhao Lili, Zhang Jing, Ge Wupeng
College of Food Science and Engineering, Northwest A&F University, Xianyang 712100, China.
School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, China.
Antioxidants (Basel). 2025 May 1;14(5):547. doi: 10.3390/antiox14050547.
This study utilized a high-fat diet-induced obese male C57BL/6 mice model to investigate the anti-obesity and lipid-lowering effects of subsp. LL-1 and LP-16. A gut microbiota analysis via 16S rRNA sequencing, along with measurements of body weight, lipids, inflammation markers, and gut metabolites, revealed that lactic acid bacteria (LAB) significantly reduced body weight, blood lipid levels, and liver oxidative stress. They also enhanced gut microbiota diversity and evenness, potentially by modulating the / ratio to limit excess energy absorption. Malondialdehyde (MDA) showed extremely significant positive correlations with , , and , and a significant positive correlation with while superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) exhibited opposite trends. Specifically, , , and showed negative correlations with MDA levels and positive correlations with SOD and GSH-Px. Short-chain fatty acids (SCFAs) positively correlated with , , , and but negatively correlated with , , , , and . They increased SCFA levels by promoting beneficial bacteria and reducing pathogens, alleviating obesity and hyperlipidemia. Additionally, they regulated the gut microbiota, decreasing bile acids and long-chain fatty acids while increasing SCFAs, short peptides, and vitamins, thereby improving gut metabolic disorders and enhancing host gut health.
本研究利用高脂饮食诱导的肥胖雄性C57BL/6小鼠模型，研究LL-1亚种和LP-16的抗肥胖和降脂作用。通过16S rRNA测序进行肠道微生物群分析，同时测量体重、脂质、炎症标志物和肠道代谢物，结果显示乳酸菌（LAB）显著降低了体重、血脂水平和肝脏氧化应激。它们还增强了肠道微生物群的多样性和均匀度，可能是通过调节/比值来限制过多的能量吸收。丙二醛（MDA）与、和呈极显著正相关，与呈显著正相关，而超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）则呈现相反趋势。具体而言，、和与MDA水平呈负相关，与SOD和GSH-Px呈正相关。短链脂肪酸（SCFA）与、、和呈正相关，但与、、、和呈负相关。它们通过促进有益菌和减少病原菌来提高SCFA水平，减轻肥胖和高脂血症。此外，它们调节肠道微生物群，减少胆汁酸和长链脂肪酸，同时增加SCFA、短肽和维生素，从而改善肠道代谢紊乱，增强宿主肠道健康。
