Liu Xin, Wu Jun, Peng Yixiu, Liu Guangzheng, Jin Kai, Niu Yingjie, Song Jiuzhou, Han Wei, Chen Guohong, Li Bichun, Zuo Qisheng
Key Laboratory of Animal Genetics, Breeding and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China.
Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou 225009, China.
Genes (Basel). 2025 Apr 24;16(5):481. doi: 10.3390/genes16050481.
Chicken Primordial Germ Cells (PGCs) are one of the few germ cells that can be cultured for a long time in vitro, but challenges remain such as low culture efficiency and unclear roles of nutrient factors and signaling pathways.
In this study, protein kinase B (AKT) pathway activator insulin-like growth factor 1 (IGF-1) was screened for its ability to promote cell proliferation by transcriptome results using various inhibitors of pathway activation. The effects of IGF-1 on PGCs were evaluated through EdU assays, qRT-PCR, flow cytometry, and migration experiments.
This study systematically examined the effects of insulin and IGF-1 on the proliferation, cell cycle, ferroptosis, migration capacity, and establishment efficiency of PGCs. The findings demonstrated that IGF-1 exhibited comparable effects to insulin and could effectively replace insulin in PGC culture systems.
The research results are expected to provide a solid theoretical basis for optimizing the chicken PGC cultivation system and promoting its practical application.
鸡原始生殖细胞（PGCs）是少数能够在体外长期培养的生殖细胞之一，但仍存在一些挑战，如培养效率低以及营养因子和信号通路的作用尚不清楚。
在本研究中，通过使用各种通路激活抑制剂的转录组结果，筛选蛋白激酶B（AKT）通路激活剂胰岛素样生长因子1（IGF-1）促进细胞增殖的能力。通过EdU检测、qRT-PCR、流式细胞术和迁移实验评估IGF-1对PGCs的影响。
本研究系统地研究了胰岛素和IGF-1对PGCs增殖、细胞周期、铁死亡、迁移能力和建立效率的影响。研究结果表明，IGF-1与胰岛素具有相似的作用，并且可以在PGC培养系统中有效替代胰岛素。
该研究结果有望为优化鸡PGC培养系统并促进其实际应用提供坚实的理论基础。
