• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

IGF1 敲低通过 ROS 依赖性 FOXO 激活引起的能量代谢功能障碍阻碍鸡心脏的心肌发育。

IGF1 Knockdown Hinders Myocardial Development through Energy Metabolism Dysfunction Caused by ROS-Dependent FOXO Activation in the Chicken Heart.

机构信息

College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, China.

College of Animal Science and Technology, Northeast Agricultural University, Harbin 150030, China.

出版信息

Oxid Med Cell Longev. 2019 Dec 24;2019:7838754. doi: 10.1155/2019/7838754. eCollection 2019.

DOI:10.1155/2019/7838754
PMID:31949883
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6948330/
Abstract

Insulin-like growth factor 1 (IGF1) is a multifunctional cellular regulatory factor that can regulate cell growth and development by mediating growth hormone stimulation. However, the mechanism of IGF1 dysfunction in cardiomyocyte development is seldom reported. To study this, we employed the models of IGF1 knockdown in chicken embryo in vivo and in cardiomyocytes in vitro. We detected the antioxidant capacity, PI3K/Akt pathway, energy metabolism-related genes, and myocardial development-related genes. Our results revealed that the low expression of IGF1 can significantly suppress the antioxidant capacity and increase the ROS ( < 0.05) levels, activating the AMPK and PI3K pathway by inhibiting the expression of IRS1. We also found that myocardial energy metabolism is blocked through IGF1, GLUT, and IGFBP inhibition, further inducing myocardial developmental disorder by inhibiting Mesp1, GATA, Nkx2.5, and MyoD expression. Altogether, we conclude that low IGF1 expression can hinder myocardial development through the dysfunction of energy metabolism caused by ROS-dependent FOXO activation.

摘要

胰岛素样生长因子 1（IGF1）是一种多功能的细胞调节因子，通过介导生长激素刺激来调节细胞的生长和发育。然而，IGF1 在心细胞发育中功能障碍的机制很少有报道。为了研究这一点，我们在体内鸡胚和体外心肌细胞中采用 IGF1 敲低模型。我们检测了抗氧化能力、PI3K/Akt 通路、能量代谢相关基因和心肌发育相关基因。我们的结果表明，IGF1 的低表达可显著抑制抗氧化能力并增加 ROS（<0.05）水平，通过抑制 IRS1 的表达来激活 AMPK 和 PI3K 通路。我们还发现，通过抑制 IGF1、GLUT 和 IGFBP 的表达，心肌能量代谢被阻断，进一步通过抑制 Mesp1、GATA、Nkx2.5 和 MyoD 的表达，诱导心肌发育障碍。总之，我们得出结论，低 IGF1 表达可通过 ROS 依赖性 FOXO 激活引起的能量代谢功能障碍阻碍心肌发育。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fb1/6948330/9db64550acf6/OMCL2019-7838754.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fb1/6948330/6b7b517486ed/OMCL2019-7838754.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fb1/6948330/76fcae085f32/OMCL2019-7838754.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fb1/6948330/aeaa3363507d/OMCL2019-7838754.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fb1/6948330/afa2fb2f8eec/OMCL2019-7838754.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fb1/6948330/1d5f7be614ad/OMCL2019-7838754.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fb1/6948330/0f848750b060/OMCL2019-7838754.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fb1/6948330/b7273b0e3d18/OMCL2019-7838754.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fb1/6948330/9db64550acf6/OMCL2019-7838754.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fb1/6948330/6b7b517486ed/OMCL2019-7838754.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fb1/6948330/76fcae085f32/OMCL2019-7838754.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fb1/6948330/aeaa3363507d/OMCL2019-7838754.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fb1/6948330/afa2fb2f8eec/OMCL2019-7838754.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fb1/6948330/1d5f7be614ad/OMCL2019-7838754.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fb1/6948330/0f848750b060/OMCL2019-7838754.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fb1/6948330/b7273b0e3d18/OMCL2019-7838754.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8fb1/6948330/9db64550acf6/OMCL2019-7838754.008.jpg

相似文献

1
IGF1 Knockdown Hinders Myocardial Development through Energy Metabolism Dysfunction Caused by ROS-Dependent FOXO Activation in the Chicken Heart.IGF1 敲低通过 ROS 依赖性 FOXO 激活引起的能量代谢功能障碍阻碍鸡心脏的心肌发育。
Oxid Med Cell Longev. 2019 Dec 24;2019:7838754. doi: 10.1155/2019/7838754. eCollection 2019.
2
Selenium deficiency-induced thioredoxin suppression and thioredoxin knock down disbalanced insulin responsiveness in chicken cardiomyocytes through PI3K/Akt pathway inhibition.硒缺乏诱导的硫氧还蛋白抑制和硫氧还蛋白敲低通过抑制 PI3K/Akt 通路使鸡心肌细胞的胰岛素反应失衡。
Cell Signal. 2017 Oct;38:192-200. doi: 10.1016/j.cellsig.2017.07.012. Epub 2017 Jul 19.
3
Regulation of cardiomyocyte proliferation and myocardial growth during development by FOXO transcription factors.FOXO转录因子对发育过程中心肌细胞增殖和心肌生长的调控。
Circ Res. 2008 Mar 28;102(6):686-94. doi: 10.1161/CIRCRESAHA.107.163428. Epub 2008 Jan 24.
4
ROS constitute a convergence nexus in the development of IGF1 resistance and impaired wound healing in a rat model of type 2 diabetes.活性氧簇在 2 型糖尿病大鼠模型中 IGF1 抵抗和伤口愈合受损的发展中构成一个汇聚枢纽。
Dis Model Mech. 2012 May;5(3):375-88. doi: 10.1242/dmm.007872. Epub 2012 Feb 23.
5
IGF1 suppresses cholesterol accumulation in the liver of growth hormone-deficient mice via the activation of ABCA1.IGF1 通过激活 ABCA1 抑制生长激素缺乏小鼠肝脏中的胆固醇积累。
Am J Physiol Endocrinol Metab. 2018 Dec 1;315(6):E1232-E1241. doi: 10.1152/ajpendo.00134.2018. Epub 2018 Aug 21.
6
Salidroside suppressing LPS-induced myocardial injury by inhibiting ROS-mediated PI3K/Akt/mTOR pathway in vitro and in vivo.红景天苷通过抑制 ROS 介导的 PI3K/Akt/mTOR 通路在体外和体内抑制 LPS 诱导的心肌损伤。
J Cell Mol Med. 2017 Dec;21(12):3178-3189. doi: 10.1111/jcmm.12871. Epub 2017 Sep 14.
7
IGF1 induces up-regulation of steroidogenic and apoptotic regulatory genes via activation of phosphatidylinositol-dependent kinase/AKT in bovine granulosa cells.IGF1 通过激活牛颗粒细胞中的磷脂酰肌醇依赖性激酶/AKT 诱导甾体生成和凋亡调节基因的上调。
Reproduction. 2010 Jan;139(1):139-51. doi: 10.1530/REP-09-0050.
8
FoxO proteins' nuclear retention and BH3-only protein Bim induction evoke mitochondrial dysfunction-mediated apoptosis in berberine-treated HepG2 cells.小檗碱处理的HepG2细胞中，FoxO蛋白的核内滞留和仅含BH3结构域蛋白Bim的诱导引发线粒体功能障碍介导的细胞凋亡。
Free Radic Biol Med. 2014 Nov;76:185-99. doi: 10.1016/j.freeradbiomed.2014.07.039. Epub 2014 Aug 13.
9
FoxO1 and FoxM1 transcription factors have antagonistic functions in neonatal cardiomyocyte cell-cycle withdrawal and IGF1 gene regulation.FoxO1 和 FoxM1 转录因子在新生儿心肌细胞细胞周期退出和 IGF1 基因调控中具有拮抗作用。
Circ Res. 2013 Jan 18;112(2):267-77. doi: 10.1161/CIRCRESAHA.112.277442. Epub 2012 Nov 14.
10
The reciprocal stability of FOXO1 and IRS2 creates a regulatory circuit that controls insulin signaling.FOXO1和IRS2的相互稳定性形成了一个控制胰岛素信号传导的调节回路。
Mol Endocrinol. 2006 Dec;20(12):3389-99. doi: 10.1210/me.2006-0092. Epub 2006 Aug 17.

引用本文的文献

1
FBXL3 serves as a suppressor of regenerative myogenesis.FBXL3作为再生性肌生成的抑制因子。
Front Immunol. 2025 Jul 18;16:1575712. doi: 10.3389/fimmu.2025.1575712. eCollection 2025.
2
Functional Equivalence of Insulin and IGF-1 in the In Vitro Culture of Chicken Primordial Germ Cells.胰岛素与胰岛素样生长因子-1在鸡原始生殖细胞体外培养中的功能等效性
Genes (Basel). 2025 Apr 24;16(5):481. doi: 10.3390/genes16050481.
3
Stem Cells Associated with Adult Skeletal Muscle Can Form Beating Cardiac Tissue In Vitro in Response to Media Containing Heparin, Dexamethasone, Growth Factors and Hydrogen Peroxide.

本文引用的文献

1
Zinc alleviates arsenism in common carp: Varied change profiles of cytokines and tight junction proteins among two intestinal segments.锌缓解鲤鱼砷中毒：两种肠道段细胞因子和紧密连接蛋白的变化特征。
Fish Shellfish Immunol. 2019 Nov;94:761-768. doi: 10.1016/j.fsi.2019.09.069. Epub 2019 Oct 1.
2
Targeting the miR-122/PKM2 autophagy axis relieves arsenic stress.靶向miR-122/PKM2自噬轴可缓解砷胁迫。
J Hazard Mater. 2020 Feb 5;383:121217. doi: 10.1016/j.jhazmat.2019.121217. Epub 2019 Sep 12.
3
lnc-3215 Suppression Leads to Calcium Overload in Selenium Deficiency-Induced Chicken Heart Lesion via the lnc-3215-miR-1594-TNN2 Pathway.
与成年骨骼肌相关的干细胞在含有肝素、地塞米松、生长因子和过氧化氢的培养基作用下可在体外形成跳动的心脏组织。
Int J Mol Sci. 2025 Mar 17;26(6):2683. doi: 10.3390/ijms26062683.
4
Andrews root extract ameliorates photoaging via regulating IRS1/PI3K/FOXO pathway.安德鲁斯根提取物通过调节IRS1/PI3K/FOXO信号通路改善光老化。
Front Pharmacol. 2025 Mar 6;16:1520392. doi: 10.3389/fphar.2025.1520392. eCollection 2025.
5
Cardioprotective mechanism of Qixuan Yijianing formula in Graves' disease mice using miRNA sequencing approach.基于miRNA测序法探讨芪玄益甲宁方对Graves病小鼠的心脏保护机制
J Tradit Chin Med. 2024 Dec;44(6):1127-1136. doi: 10.19852/j.cnki.jtcm.20240927.005.
6
Autophagy and the pancreas: Healthy and disease states.自噬与胰腺：健康与疾病状态
Front Cell Dev Biol. 2024 Sep 24;12:1460616. doi: 10.3389/fcell.2024.1460616. eCollection 2024.
7
In-depth transcriptome profiling of Cherry Valley duck lungs exposed to chronic heat stress.对暴露于慢性热应激的樱桃谷鸭肺进行深度转录组分析。
Front Vet Sci. 2024 Jul 22;11:1417244. doi: 10.3389/fvets.2024.1417244. eCollection 2024.
8
Differentiation of Pluripotent Stem Cells for Disease Modeling: Learning from Heart Development.用于疾病建模的多能干细胞分化：从心脏发育中汲取经验。
Pharmaceuticals (Basel). 2024 Mar 5;17(3):337. doi: 10.3390/ph17030337.
9
Synergistic effects of hormones on structural and functional maturation of cardiomyocytes and implications for heart regeneration.激素对心肌细胞结构和功能成熟的协同作用及其对心脏再生的影响。
Cell Mol Life Sci. 2023 Aug 5;80(8):240. doi: 10.1007/s00018-023-04894-6.
10
LINC00638 promotes the progression of non-small cell lung cancer by regulating the miR-541-3p/IRS1/PI3K/Akt axis.LINC00638通过调控miR-541-3p/胰岛素受体底物1/磷脂酰肌醇-3-激酶/蛋白激酶B轴促进非小细胞肺癌进展。
Heliyon. 2023 Jun 15;9(6):e16999. doi: 10.1016/j.heliyon.2023.e16999. eCollection 2023 Jun.
lnc-3215抑制通过lnc-3215-miR-1594-TNN2途径导致硒缺乏诱导的鸡心脏病变中的钙超载。
Mol Ther Nucleic Acids. 2019 Dec 6;18:1-15. doi: 10.1016/j.omtn.2019.08.003. Epub 2019 Aug 12.
4
Role of miR-731 and miR-2188-3p in mediating chlorpyrifos induced head kidney injury in common carp via targeting TLR and apoptosis pathways.miR-731 和 miR-2188-3p 通过靶向 TLR 和凋亡途径介导毒死蜱诱导鲤鱼头部肾脏损伤的作用。
Aquat Toxicol. 2019 Oct;215:105286. doi: 10.1016/j.aquatox.2019.105286. Epub 2019 Aug 27.
5
Application of transcriptome analysis: Oxidative stress, inflammation and microtubule activity disorder caused by ammonia exposure may be the primary factors of intestinal microvilli deficiency in chicken.转录组分析的应用：氨暴露引起的氧化应激、炎症和微管活性紊乱可能是鸡肠道微绒毛缺失的主要因素。
Sci Total Environ. 2019 Dec 15;696:134035. doi: 10.1016/j.scitotenv.2019.134035. Epub 2019 Aug 21.
6
Hydrogen sulfide-induced oxidative stress leads to excessive mitochondrial fission to activate apoptosis in broiler myocardia.硫化氢诱导的氧化应激导致过度的线粒体分裂，从而激活肉鸡心肌细胞凋亡。
Ecotoxicol Environ Saf. 2019 Nov 15;183:109578. doi: 10.1016/j.ecoenv.2019.109578. Epub 2019 Aug 20.
7
HS promotes a glycometabolism disorder by disturbing the Th1/Th2 balance during LPS-induced inflammation in the skeletal muscles of chickens.HS 通过在 LPS 诱导的鸡骨骼肌炎症期间干扰 Th1/Th2 平衡来促进糖代谢紊乱。
Chemosphere. 2019 May;222:124-131. doi: 10.1016/j.chemosphere.2019.01.136. Epub 2019 Jan 23.
8
Hydrogen sulfide exposure triggers chicken trachea inflammatory injury through oxidative stress-mediated FOS/IL8 signaling.硫化氢暴露通过氧化应激介导的 FOS/IL8 信号触发鸡气管炎症损伤。
J Hazard Mater. 2019 Apr 15;368:243-254. doi: 10.1016/j.jhazmat.2019.01.054. Epub 2019 Jan 22.
9
Arsenite renal apoptotic effects in chickens co-aggravated by oxidative stress and inflammatory response.砷酸盐诱导的肾细胞凋亡效应在氧化应激和炎症反应共同作用下加剧。
Metallomics. 2018 Dec 12;10(12):1805-1813. doi: 10.1039/c8mt00234g.
10
Effect of Gpx3 gene silencing by siRNA on apoptosis and autophagy in chicken cardiomyocytes.siRNA 沉默 Gpx3 基因对鸡心肌细胞凋亡和自噬的影响。
J Cell Physiol. 2019 Jun;234(6):7828-7838. doi: 10.1002/jcp.27842. Epub 2018 Dec 4.