Zheng Yuling, Wang Shanshan, He Ling, Chen Rui, Zhang Wei, He Huachuan, Hu Hanbing, Liu Xiaoyun, Wan Heping, Wu Chunhong
Hubei Engineering Research Center for Protection and Utilization of Special Biological Resources in the Hanjiang River Basin, College of Life Science, Jianghan University, Wuhan 430056, China.
Genes (Basel). 2025 May 7;16(5):559. doi: 10.3390/genes16050559.
BACKGROUND/OBJECTIVES: Abiotic stresses severely constrain the yield of and aldehyde dehydrogenases (ALDHs) play a pivotal role in plant stress resistance. This study aims to systematically identify the gene family members in and elucidate their expression patterns under salt and drought stress.
Using the Arabidopsis thaliana AtALDH proteins as seed sequences, BLASTp alignment was performed against the whole-protein sequence database, combined with the conserved domain PF00171 of the ALDH proteins. A total of 39 gene family members were identified, and their physicochemical properties, structures, phylogenetic relationships, interspecies collinearity, and intraspecies collinearity were analyzed. The qRT-PCR method was employed to quantify the relative expression levels of the genes potentially associated with stress resistance under various treatments, and their effects on drought and salt stress tolerance were evaluated.
The results demonstrated that were universally significantly upregulated under salt stress, while exhibiting predominantly upregulated trends under drought stress. These findings suggest that may enhance plant resistance to both salt and drought stress by modulating the aldehyde metabolic pathways.
This study provides a theoretical basis for elucidating the functional roles and molecular genetic mechanisms of the gene family in under salt and drought stress.
背景/目的：非生物胁迫严重限制植物产量，醛脱氢酶（ALDHs）在植物抗逆性中起关键作用。本研究旨在系统鉴定植物中的ALDH基因家族成员，并阐明其在盐胁迫和干旱胁迫下的表达模式。
以拟南芥AtALDH蛋白为种子序列，对植物全蛋白序列数据库进行BLASTp比对，并结合ALDH蛋白的保守结构域PF00171。共鉴定出39个ALDH基因家族成员，并分析了它们的理化性质、结构、系统发育关系、种间共线性和种内共线性。采用qRT-PCR方法定量分析不同处理下可能与抗逆性相关的ALDH基因的相对表达水平，并评估它们对干旱和盐胁迫耐受性的影响。
结果表明，ALDH在盐胁迫下普遍显著上调，而在干旱胁迫下主要呈上调趋势。这些发现表明，ALDH可能通过调节醛代谢途径增强植物对盐胁迫和干旱胁迫的抗性。
本研究为阐明盐胁迫和干旱胁迫下植物中ALDH基因家族的功能作用和分子遗传机制提供了理论依据。
