Vigni Giulio Edoardo, Licciardi Mariano, D'itri Lorenzo, Terracina Francesca, Scirè Sergio, Arrabito Giuseppe, Pignataro Bruno, Camarda Lawrence, Cassata Giovanni, Puleio Roberto, Di Silvestre Lucio, Cicero Luca
Department of Orthopaedics and Traumatology, University of Palermo, 90133 Palermo, Italy.
Department of Biological, Chemical, and Pharmaceutical Sciences and Technologies (STEBICEF), University of Palermo, Via Archirafi 30, 90132 Palermo, Italy.
Materials (Basel). 2025 May 12;18(10):2234. doi: 10.3390/ma18102234.
Extensive bone loss represents a great challenge for orthopedic and reconstructive surgery. On an in vivo rabbit model, the healing of two bone defects on a long bone, tibia, was studied. A polybutylene succinate (PBS) microfibrillar scaffold was implemented with BMP-2 protein and hydroxyapatite (HA) as potential osteogenic factors. The present study was carried out on 6 male New Zealand white (4-6 months old) rabbits in vivo model. One bone defect was created in each subject on the tibia. The controls were left to heal spontaneously while the study samples were treated with the polybutylene succinate (PBS) microfibrillar scaffolds doped with BMP-2 and HA. Histological and immunohistochemical analyses were performed after euthanasia at 3 and 6 months. The bone defect treated with the BMP-2 PBS scaffold shows, from 3 months, a significantly increased presence of activated osteoblasts with mineralized bone tissue deposition. This study confirms the great potential of PBS scaffolds in the clinical treatment of bone defects.
广泛的骨质流失对骨科和重建手术来说是一个巨大的挑战。在一个体内兔模型上，研究了长骨（胫骨）上两个骨缺损的愈合情况。使用聚丁二酸丁二醇酯（PBS）微纤维支架，并将骨形态发生蛋白-2（BMP-2）和羟基磷灰石（HA）作为潜在的成骨因子。本研究在6只雄性新西兰白兔（4至6个月大）的体内模型上进行。在每个实验对象的胫骨上制造一个骨缺损。对照组任其自然愈合，而研究样本则用掺杂了BMP-2和HA的聚丁二酸丁二醇酯（PBS）微纤维支架进行治疗。在3个月和6个月安乐死后进行组织学和免疫组织化学分析。用BMP-2 PBS支架治疗的骨缺损从3个月起显示，活化成骨细胞的存在显著增加，并有矿化骨组织沉积。本研究证实了PBS支架在骨缺损临床治疗中的巨大潜力。
