Trujillo-Rodríguez Gerardo, Jiménez-Martínez Mariana Lizbeth, Flores-Contreras Elda, González Gonzalez Everardo, Ramírez Ahuja María de Lourdes, Garza Veloz Idalia, Flores Suarez Adriana E, Correa Morales Fabian, Dzul Manzanilla Felipe, Rodriguez Sanchez Iram P, Martínez Fierro Margarita L
Molecular Medicine Laboratory, Unidad Académica de Medicina Humana y C.S., Universidad Autónoma de Zacatecas, Zacatecas 98600, Zacatecas, Mexico.
Laboratorio de Fisiología Molecular y Estructural, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, Av. Universidad S/N Ciudad Universitaria, San Nicolás de los Garza 66451, Nuevo León, Mexico.
Insects. 2025 Apr 27;16(5):460. doi: 10.3390/insects16050460.
is a major vector of arboviruses, including dengue, Zika, chikungunya, yellow fever, and Mayaro. The extensive use of insecticides has led to resistance, complicating vector control efforts. MicroRNAs (miRNAs) are post-transcriptional regulators involved in detoxification and stress adaptation; however, their role in the insecticide response of remains unclear. This study analyzed miRNA expression in following imidacloprid exposure, comparing a field strain, Martinez de la Torre from Mexico (MT), and a susceptible reference strain, New Orleans (NO). Small RNA sequencing identified 96 miRNAs, with miR-1, miR-281-5p, miR-100, and miR-184 being consistently expressed across all conditions. In contrast, let-7, miR-124, and miR-13-3p were exclusively detected in wild mosquitoes exposed to imidacloprid, while miR-14 and miR-275-3p showed condition-specific expression. Distinct miRNA expression patterns were observed between the field and susceptible strains, with specific miRNAs showing condition-dependent expression. These findings suggest that certain miRNAs may serve as potential biomarkers for resistance monitoring, contributing to a better understanding of insecticide response mechanisms and informing novel vector control strategies.
是虫媒病毒的主要传播媒介，包括登革热、寨卡、基孔肯雅热、黄热病和马亚罗病毒。杀虫剂的广泛使用导致了抗药性，使病媒控制工作复杂化。微小RNA（miRNA）是参与解毒和应激适应的转录后调节因子；然而，它们在[昆虫名称未给出]对杀虫剂反应中的作用仍不清楚。本研究分析了吡虫啉暴露后[昆虫名称未给出]中的miRNA表达，比较了一个野外品系，来自墨西哥的马丁内斯·德拉托雷（MT），和一个敏感参考品系，新奥尔良（NO）。小RNA测序鉴定出96种miRNA，其中miR-1、miR-281-5p、miR-100和miR-184在所有条件下均持续表达。相比之下，let-7、miR-124和miR-13-3p仅在暴露于吡虫啉的野生蚊子中检测到，而miR-14和miR-275-3p表现出条件特异性表达。在野外品系和敏感品系之间观察到不同的miRNA表达模式，特定的miRNA表现出条件依赖性表达。这些发现表明，某些miRNA可能作为抗药性监测的潜在生物标志物，有助于更好地理解杀虫剂反应机制，并为新的病媒控制策略提供信息。
