Majchrowska-Safaryan Anna, Różalska Sylwia, Tkaczuk Cezary, Nowak Monika
Faculty of Agricultural Sciences, Institute of Agriculture and Horticulture, University of Siedlce, 08-110 Siedlce, Poland.
Department of Industrial Microbiology and Biotechnology, Faculty of Biology and Environmental Protection, University of Lodz, 90-136 Lodz, Poland.
Insects. 2025 May 18;16(5):533. doi: 10.3390/insects16050533.
Pyrethroids are chemical insecticides used on a large scale in agriculture, horticulture, and forest protection. In order to reduce their use in IPM, alternative methods of controlling insect pests are introduced, such as the use of biopesticides based on entomopathogenic fungi (EPF). Species of the genus are characterized by a very broad spectrum of action, which is why they are often used to produce preparations based on EPF. The aim of the study was to determine the effect of different doses of tested pyrethroids on the colony growth and metabolic activity of EPF from the genus. In vitro, the effect of three pyrethroids (deltamethrin, λ-cyhalothrin, and α-cypermethrin) added to SDA medium at a dose 10 times lower than the recommended field dose (A), the recommended field dose (B), and 10 times higher than the recommended field dose (C) on colony growth and metabolic activity of and was tested. The research carried out showed that pyrethroid insecticides used in the experiment showed various toxic effects towards the tested EPF of the genus The studies conducted showed that on the 20th day of the observation, λ-cyhalothrin used in the recommended field dose limited the growth of to the least extent in relation to the other tested pyrethroids. However, with respect to the fungus , no toxic effect of this pyrethroid was found. Based on the results obtained, it was found that λ-cyhalothrin used in the recommended field dose and 10 times lower than recommended significantly increased the metabolic activity of . In relation to the strain, detlamethrin used in each of the tested concentrations significantly affected its viability.
拟除虫菊酯是大规模用于农业、园艺和森林保护的化学杀虫剂。为了减少其在综合虫害管理中的使用，引入了控制害虫的替代方法，例如使用基于昆虫病原真菌（EPF）的生物农药。该属的物种具有非常广泛的作用谱，这就是它们经常被用于生产基于EPF的制剂的原因。本研究的目的是确定不同剂量的受试拟除虫菊酯对该属EPF菌落生长和代谢活性的影响。在体外，测试了三种拟除虫菊酯（溴氰菊酯、高效氯氟氰菊酯和氯氰菊酯）以低于推荐田间剂量10倍（A）、推荐田间剂量（B）和高于推荐田间剂量1（C）添加到SDA培养基中对 和 的菌落生长和代谢活性的影响。所进行的研究表明，实验中使用的拟除虫菊酯杀虫剂对受试的该属EPF表现出各种毒性作用。所进行的研究表明，在观察的第20天，与其他受试拟除虫菊酯相比，以推荐田间剂量使用的高效氯氟氰菊酯对 的生长限制最小。然而，对于真菌 ，未发现该拟除虫菊酯的毒性作用。基于获得的结果，发现以推荐田间剂量和低于推荐剂量10倍使用的高效氯氟氰菊酯显著提高了 的代谢活性。对于 菌株，在每个测试浓度下使用的溴氰菊酯均显著影响其活力。
