Ichikawa K, Hashizume K, Kobayashi M, Yamada T
Endocrinology. 1985 Nov;117(5):1749-58. doi: 10.1210/endo-117-5-1749.
The effect of thyroid hormone on the generation of modulators of mitochondrial protein synthesis was investigated. The modulators were present in the 150,000 X g supernatant (S-150) prepared from Wistar rat liver and kidney. In young rats (50 g BW), a stimulator was found in liver, and an inhibitory modulator was present in kidney tissue. Generation of these modulators was stimulated by T4 administration to the animals. In aged rats (200 g BW), the production of an inhibitory modulator in both liver and kidney was stimulated by thyroid hormone administration. Both the stimulatory and inhibitory modulators were inactivated by pretreatment with trypsin. The mol wt of these modulators was estimated by gel filtration study, and for both the stimulatory and inhibitory modulator proteins was approximately 10,000. The results suggested that thyroid hormone regulates mitochondrial protein synthesis through the stimulation of synthesis of mitochondrial protein synthesis modulators and that the tissue-specific modulators (stimulatory in liver and inhibitory in kidney) can be produced in young animals. In aged animals, however, it is postulated that thyroid hormone stimulates biosynthesis of an inhibitory modulator in both liver and kidney.
研究了甲状腺激素对线粒体蛋白质合成调节剂生成的影响。这些调节剂存在于从Wistar大鼠肝脏和肾脏制备的150,000×g上清液（S-150）中。在幼鼠（体重50克）中，肝脏中发现一种刺激剂，肾脏组织中存在一种抑制性调节剂。给动物注射T4可刺激这些调节剂的生成。在老年大鼠（体重200克）中，给甲状腺激素可刺激肝脏和肾脏中抑制性调节剂的产生。刺激型和抑制型调节剂经胰蛋白酶预处理后均失活。通过凝胶过滤研究估计了这些调节剂的分子量，刺激型和抑制型调节蛋白的分子量均约为10,000。结果表明，甲状腺激素通过刺激线粒体蛋白质合成调节剂的合成来调节线粒体蛋白质合成，并且组织特异性调节剂（肝脏中为刺激型，肾脏中为抑制型）可在幼龄动物中产生。然而，据推测，在老年动物中，甲状腺激素会刺激肝脏和肾脏中抑制性调节剂的生物合成。
