Silberzahn P, Dehennin L, Zwain I, Reiffsteck A
Endocrinology. 1985 Nov;117(5):2176-81. doi: 10.1210/endo-117-5-2176.
Follicular fluid was obtained from equine follicles at different stages of development as determined by ultrastructural study. Gas chromatography-mass spectrometry associated with stable isotope dilution permitted the demonstration of high levels of 4-estrene-3,7-dione and 17 beta-hydroxy-4-estren-3-one, 17 beta-hydroxy-4-estren-3-one levels often being about 10 times higher than those of testosterone. These findings suggest that in the mare ovary, an aromatizing pathway may proceed using these 19-norsteroids as intermediates. As a consequence of this high level of 19-norsteroids, testosterone content may be most often overestimated by RIA. RIA of steroids in follicular fluid should be validated by a reference technique based on gas chromatography-mass spectrometry, associated with stable isotope dilution.
通过超微结构研究确定，从处于不同发育阶段的马卵泡中获取卵泡液。气相色谱 - 质谱联用稳定同位素稀释法可检测到高水平的4 - 雌烯 - 3,7 - 二酮和17β - 羟基 - 4 - 雌烯 - 3 - 酮，17β - 羟基 - 4 - 雌烯 - 3 - 酮的水平通常比睾酮高约10倍。这些发现表明，在母马卵巢中，可能存在一条以这些19 - 去甲甾体为中间体的芳香化途径。由于19 - 去甲甾体水平较高，放射免疫分析法（RIA）测定的睾酮含量可能经常被高估。卵泡液中类固醇的放射免疫分析法应通过基于气相色谱 - 质谱联用稳定同位素稀释的参考技术进行验证。
