Silberzahn P, Almahbobi G, Dehennin L, Merouane A
J Steroid Biochem. 1985 Apr;22(4):501-5. doi: 10.1016/0022-4731(85)90169-4.
Equine follicular fluid was aspirated at various developmental stages (viable, preovulatory and atretic) determined by ultrastructural study. Estrogens and progestins were analyzed by gas chromatography-mass spectrometry associated with stable isotope dilution. Progesterone and 17-hydroxyprogesterone were the principal progestins of the preovulatory and viable follicles. Among the catechol estrogens, 2-hydroxy-estradiol was particularly abundant in the preovulatory follicle and its definitive identification was made by the scan of a full mass spectrum.
通过超微结构研究确定了处于不同发育阶段（可存活、排卵前和闭锁）的马卵泡液，并进行抽吸。采用与稳定同位素稀释联用的气相色谱 - 质谱法分析雌激素和孕激素。孕酮和17 - 羟孕酮是排卵前和可存活卵泡的主要孕激素。在儿茶酚雌激素中，2 - 羟基雌二醇在排卵前卵泡中特别丰富，通过全质谱扫描对其进行了明确鉴定。
