Zhang Yiming, Ji Yanjun, Liu Fuxin, Wang Yutong, Feng Chengyi, Zhou Zhenzhen, Zhang Zijian, Han Long, Li Jinxia, Wang Mingyu, Li Lixin
Key Laboratory of Saline-Alkali Vegetation Ecology Restoration, Ministry of Education, College of Life Sciences, Northeast Forestry University, Harbin 150040, China.
Int J Mol Sci. 2025 May 20;26(10):4919. doi: 10.3390/ijms26104919.
Aluminum toxicity severely inhibits root elongation and nutrient uptake, causing global agricultural yield losses. Dissolved Al are accumulating in plants and subsequently entering food chains via crops and forage plants. Chronic dietary exposure to Al poses a risk to human health. In this study, sp. strain ADAl3-4, isolated from plant rhizosphere soil, significantly enhanced plant development and biomass. Phenotypic validation using mutants showed that strain ADAl3-4 regulates plant growth and development under aluminum stress by reprogramming the cell cycle, regulating auxin and ion homeostasis, and enhancing the root absorption of Al from the soil. Transcriptomic and biochemical analyses showed that strain ADAl3-4 promotes plant growth via regulating signal transduction, phytohormone biosynthesis, flavonoid biosynthesis, and antioxidant capacity, etc., under aluminum stress. Our findings indicate that sp. strain ADAl3-4 enhances plant development and stress resilience under Al toxicity through a coordinated multi-dimensional regulatory network. Furthermore, strain ADAl3-4 promoted the root absorption of aluminum rather than the transportation of Al to the aerial part, endowing it with application prospects.
铝毒严重抑制根系伸长和养分吸收,导致全球农业产量损失。溶解态铝在植物中积累,随后通过作物和饲料植物进入食物链。长期膳食接触铝对人类健康构成风险。在本研究中,从植物根际土壤中分离出的sp. 菌株ADAl3-4显著促进了植物生长和生物量积累。使用突变体进行的表型验证表明,菌株ADAl3-4通过重新编程细胞周期、调节生长素和离子稳态以及增强根系对土壤中铝的吸收,在铝胁迫下调节植物生长发育。转录组学和生化分析表明,在铝胁迫下,菌株ADAl3-4通过调节信号转导、植物激素生物合成、类黄酮生物合成和抗氧化能力等促进植物生长。我们的研究结果表明,sp. 菌株ADAl3-4通过协调的多维调控网络增强了植物在铝毒下的生长发育和抗逆性。此外,菌株ADAl3-4促进了根系对铝的吸收,而不是将铝运输到地上部分,这使其具有应用前景。