Wu Shaoping, Liao Jinyan, Ye Chunxing, Chen Shanyi, Wang Yingshan, Zhang Xiaochun, Huang Junwen, Chen Cong
School of Life Sciences, Zhaoqing University, Zhaoqing 526061, China.
Life (Basel). 2025 May 19;15(5):806. doi: 10.3390/life15050806.
The physiological and biochemical responses of Blanco var. Gonggan (Gonggan) to high-temperature stress were explored in the present study, offering valuable insights into the growth of this plant in elevated temperature scenarios. Plants were exposed to daytime temperatures of 22 °C, 40 °C, and 45 °C, with corresponding nighttime temperatures of 17 °C, 35 °C, and 40 °C, respectively. Each treatment was administered for 12 h, with a daytime light intensity of 14,400 lux. Key parameters such as the chlorophyll content, peroxidase activity, malondialdehyde content, cytoplasmic membrane permeability, and photosynthetic metrics were assessed. The results showed that the content of malondialdehyde decreased with the increase in temperature, with the highest content at 22 °C. After high-temperature treatment at 40 °C and 45 °C, there was a significant difference ( < 0.05) compared with the Gonggan plants treated at 22 °C. Peroxidase activity exhibited an increasing trend as the temperature increased, and there was a significant difference ( < 0.05) between the peroxidase activity at 22 °C and 45 °C. Similar trends are displayed for high-temperature stress, stomatal conductance, transpiration rate, and intercellular CO, which initially decreased and then significantly increased. The net photosynthetic rate (Pn) showed a trend of first increasing and then decreasing. When plants were subjected to high-temperature stress at 40 °C, the net photosynthetic rate showed a significant increase compared to the control group at 22 °C, but in a 45 °C stress environment, the Pn showed a decreasing trend. In the experimental group, relative conductivity decreased with the increase in temperature. Meanwhile, Gonggan plants exhibited moderate heat tolerance to short-term or moderate high-temperature stress, primarily through antioxidant and repair mechanisms. However, their heat tolerance was limited under prolonged or extremely high-temperature conditions, characterized by significant membrane damage and photosynthetic inhibition. Overall, Gonggan plants demonstrate moderate heat tolerance, making them suitable for intermittent high-temperature environments rather than prolonged extreme heat conditions. These findings provide a foundation for understanding the adaptive strategies of Gonggan plants and their cultivation in high-temperature settings.
本研究探讨了凤梨穗龙眼（Gonggan）对高温胁迫的生理生化响应，为了解该植物在高温环境下的生长提供了有价值的见解。将植株分别置于白天温度为22℃、40℃和45℃，相应夜间温度分别为17℃、35℃和40℃的环境中。每个处理持续12小时，白天光照强度为14400勒克斯。评估了叶绿素含量、过氧化物酶活性、丙二醛含量、细胞质膜透性和光合指标等关键参数。结果表明，丙二醛含量随温度升高而降低，在22℃时含量最高。在40℃和45℃高温处理后，与22℃处理的凤梨穗龙眼植株相比，差异显著（<0.05）。过氧化物酶活性随温度升高呈上升趋势，22℃和45℃时的过氧化物酶活性差异显著（<0.05）。高温胁迫、气孔导度、蒸腾速率和胞间CO也呈现类似趋势，最初下降，然后显著增加。净光合速率（Pn）呈先增加后降低的趋势。当植株在40℃下遭受高温胁迫时，净光合速率与22℃对照组相比显著增加，但在45℃胁迫环境下，Pn呈下降趋势。在实验组中，相对电导率随温度升高而降低。同时，凤梨穗龙眼植株对短期或中度高温胁迫表现出中等耐热性，主要通过抗氧化和修复机制。然而，在长期或极端高温条件下，它们的耐热性有限，表现为明显的膜损伤和光合抑制。总体而言，凤梨穗龙眼植株表现出中等耐热性，使其适合于间歇性高温环境，而非长期极端高温条件。这些发现为理解凤梨穗龙眼植株的适应策略及其在高温环境中的栽培提供了基础。
