Mohamud Dirie Layla, Yurdakul Tahire, Isik Sevim, Tarbiat Shirin
Graduate School of Science, Department of Molecular Biology, Uskudar University, 34662 Istanbul, Turkey.
Stem Cell Research and Application Center (USKOKMER), Uskudar University, 34662 Istanbul, Turkey.
Molecules. 2025 May 16;30(10):2187. doi: 10.3390/molecules30102187.
Celery ( L.), one of the numerous members of the Apiaceae family, has been traditionally used as food and medicine due to its nutraceutical properties. Nevertheless, understanding the neuroprotective effects of this species requires evaluation through different mechanisms relevant to Alzheimer's disease (AD) treatment. This study explored the neuroprotective potential of ethanolic extracts of celery leaves. Liquid chromatography and mass spectrometry-based metabolomics analysis of the extract revealed the existence of a diverse array of secondary metabolites, including phenolic acids, hydroxycinnamic acid, flavonoids, flavonoid -glycosides, flavonol, glycosides, and isoflavones. Celery extract protects human neuroblastoma SH-SY5Y cells against 15 µM amyloid-beta (Aβ) toxicity, enhancing their vitality from 67% to 81.74% at 100 µg/mL. The extract inhibited the enzymes associated with AD, including acetylcholinesterase (AChE), butyrylcholinesterase (BChE), glycogen synthase kinase 3 beta (GSK3β), cyclooxygenase 1 (COX-1), and cyclooxygenase 2 (COX-2) with IC values of 21.84, 61.27, 45.94, 34.1, and 52.2 µg/mL, respectively. In conclusion, celery leaf extract components may be potential therapeutic candidates for AD prevention and treatment.
芹菜（Apium graveolens L.）是伞形科众多成员之一，由于其营养保健特性，传统上一直被用作食物和药物。然而，要了解该物种的神经保护作用，需要通过与阿尔茨海默病（AD）治疗相关的不同机制进行评估。本研究探讨了芹菜叶乙醇提取物的神经保护潜力。对该提取物进行基于液相色谱和质谱的代谢组学分析，发现存在多种次生代谢产物，包括酚酸、羟基肉桂酸、黄酮类、黄酮糖苷、黄酮醇、糖苷和异黄酮。芹菜提取物可保护人神经母细胞瘤SH-SY5Y细胞免受15 μM淀粉样β蛋白（Aβ）毒性的影响，在100 μg/mL时将其活力从�7%提高到81.74%。该提取物抑制了与AD相关的酶，包括乙酰胆碱酯酶（AChE）、丁酰胆碱酯酶（BChE）、糖原合酶激酶3β（GSK3β）、环氧化酶1（COX-1）和环氧化酶2（COX-2），其IC值分别为21.84、61.27、45.9、34.1和52.2 μg/mL。总之，芹菜叶提取物成分可能是预防和治疗AD的潜在候选治疗药物。
