Zhautikova Saule B, Abdykhanova Nursipat N, Fedorishin Dmitry A, Shapovalova Yelena G, Khlebnikov Andrei I, Bakibaev Abdigali A, Kurzina Irina A, Kabieva Saule K, Boranbay Nazerke, Zhumanazarova Gaziza M
Karaganda Medical University, Karaganda 100900, Kazakhstan.
Karaganda Industrial University, Temirtau 101400, Kazakhstan.
Molecules. 2025 May 21;30(10):2249. doi: 10.3390/molecules30102249.
In this study, cucurbit[n]urils (n = 6, 7, 8) were carefully evaluated for their cytotoxicity and immunotoxicity to human peripheral blood monocytes. The cytotoxicity was studied by evaluating the survival of monocytes, while the immunotoxicity level was assessed by analyzing the inflammatory mediators secreted by them using an enzyme-linked immunosorbent assay. It was found that cucurbit[n]urils (n = 6, 7, 8) in the used concentration (10 M) do not cause a negative effect on cell viability, which is maintained at a level above 50%. At the same time, cucurbit[n]urils (n = 6, 7, 8) do not cause pro-inflammatory activation of monocytic macrophages. The absence of stimulation of pro-inflammatory cytokine expression demonstrates the promising biocompatibility of the studied compounds, which is crucial for their successful clinical use. The obtained results of molecular modeling show the possibility of formation of , , and associates with various Toll-like receptors, which also confirms good prospects for the development of new ways of medical application of cucurbit[n]urils.
在本研究中，对葫芦[n]脲（n = 6、7、8）对人外周血单核细胞的细胞毒性和免疫毒性进行了仔细评估。通过评估单核细胞的存活率来研究细胞毒性，而通过使用酶联免疫吸附测定法分析它们分泌的炎症介质来评估免疫毒性水平。结果发现，所用浓度（10 μM）的葫芦[n]脲（n = 6、7、8）对细胞活力没有负面影响，细胞活力维持在50%以上的水平。同时，葫芦[n]脲（n = 6、7、8）不会引起单核巨噬细胞的促炎激活。促炎细胞因子表达未受刺激表明所研究化合物具有良好的生物相容性，这对其成功临床应用至关重要。分子模拟获得的结果表明，葫芦[n]脲有可能与各种Toll样受体形成 、 和 缔合物，这也证实了开发葫芦[n]脲医学应用新方法的良好前景。
