Vuković Dragana, Miletić Maja, Toljić Boško, Milojević Nikola, Jovanović Olivera, Kuzmanović Pfićer Jovana, Škoro Nikola, Puač Nevena
Faculty of Medicine, University of Belgrade, Dr Subotića starijeg 8, 11000 Belgrade, Serbia.
School of Dental Medicine, University of Belgrade, Dr Subotića starijeg 8, 11000 Belgrade, Serbia.
Pathogens. 2025 Apr 24;14(5):410. doi: 10.3390/pathogens14050410.
The scope of the antibacterial effects of plasma-activated water (PAW) is not yet fully comprehended. We investigated the activity of PAW produced by the in-house 3-pin atmospheric pressure plasma jet against carbapenem-resistant and vancomycin-resistant , with a focus on PAW's potential to promote susceptibility to conventional antibiotics in these bacteria. Bacterial inactivation was determined by the colony count after 15 and 60 min PAW treatments. Minimum inhibitory concentrations (MICs) measured following repeated exposures to PAW across multiple generations of bacteria enabled the assessment of changes in susceptibility to antibiotics. The PAW's efficacy was also analyzed through the detection of intracellular reactive oxygen and nitrogen species in treated bacteria. Time-dependent significant inactivation efficiency against was observed (log reduction 6.92 ± 0.24 after 60 min exposure), while effects on were limited. PAW demonstrated potential to decrease the MICs of crucial antibiotics. Namely, a 50 to 62.5% decrease in the MICs of colistin against and a 25% reduction in the MICs of vancomycin against enterococci were recorded. We found a significant increase in the superoxide anion concentration in and cells after PAW treatments. This study indicates that PAW's inactivating efficacy coupled with the capacity for the potentiation of antibiotic effects is a promising combination against multidrug-resistant bacteria.
等离子体活化水（PAW）的抗菌作用范围尚未完全明确。我们研究了内部三针大气压等离子体射流产生的PAW对耐碳青霉烯类和耐万古霉素类细菌的活性，重点关注PAW促进这些细菌对传统抗生素敏感性的潜力。通过PAW处理15分钟和60分钟后的菌落计数来确定细菌的失活情况。在多代细菌中反复暴露于PAW后测量最小抑菌浓度（MIC），从而评估对抗生素敏感性的变化。还通过检测经处理细菌中的细胞内活性氧和氮物质来分析PAW的功效。观察到对[具体细菌名称1]有时间依赖性的显著失活效率（暴露60分钟后对数减少6.92±0.24），而对[具体细菌名称2]的影响有限。PAW显示出降低关键抗生素MIC的潜力。具体而言，记录到黏菌素对[具体细菌名称1]的MIC降低了50%至62.5%，万古霉素对肠球菌的MIC降低了25%。我们发现PAW处理后[具体细菌名称1]和[具体细菌名称2]细胞中的超氧阴离子浓度显著增加。这项研究表明，PAW的失活功效以及增强抗生素作用的能力是对抗多重耐药细菌的一种有前景的组合。
