Chang Ziru, Tian Xiaona, Niu Xiaocui, Bai Meiting, Bai Wei, Wang Ruigang, Li Guojing, Yang Qi
Key Laboratory of Plants Adversity Adaptation and Genetic Improvement in Cold and Arid Regions of Inner Mongolia, Inner Mongolia Agricultural University, Hohhot 010018, China.
Plant Protection Institute, Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Hohhot 010031, China.
Plants (Basel). 2025 May 19;14(10):1514. doi: 10.3390/plants14101514.
The PADRE (Pathogen and abiotic stress response, cadmium tolerance, disordered region-containing) family of genes, which contains the structural DUF4228 domain of unknown function (DUF), has been reported to be associated with plant responses to abiotic stresses. However, the specific functions of this family in the salt stress response remain unknown. is induced by salt stress and ABA (abscisic acid). After the overexpression of in Arabidopsis, seeds were found to be insensitive to ABA treatment. After salt treatment, the overexpression lines presented a significantly lower survival rate, increased MDA (Malondialdehyde) content, and reduced antioxidant enzyme activities compared with the wild-type, and were more sensitive to salt stress. Transcriptome data analysis further revealed that overexpression resulted in different degrees of down-regulation for a series of positive regulators related to ABA catabolism, transport, and their mediated plant responses to salt stress. In addition, the expression of genes related to ROS (reactive oxygen species) scavenging was down-regulated. In conclusion, AtPADRE13 plays a negative regulatory role in the response to salt stress in Arabidopsis.
PADRE（病原体与非生物胁迫响应、耐镉性、含无序区域）基因家族包含功能未知的结构域DUF4228，据报道该家族与植物对非生物胁迫的响应有关。然而，该家族在盐胁迫响应中的具体功能仍不清楚。[该基因名称缺失]受盐胁迫和脱落酸（ABA）诱导。在拟南芥中过表达[该基因名称缺失]后，发现种子对ABA处理不敏感。盐处理后，与野生型相比，过表达株系的存活率显著降低，丙二醛（MDA）含量增加，抗氧化酶活性降低，且对盐胁迫更敏感。转录组数据分析进一步表明，[该基因名称缺失]的过表达导致一系列与ABA分解代谢、转运及其介导的植物对盐胁迫响应相关的正向调节因子不同程度下调。此外，与活性氧（ROS）清除相关的基因表达也下调。总之，AtPADRE13在拟南芥对盐胁迫的响应中起负调控作用。
