First Report of Meningitis in a Non-Pregnant Adult in Italy.

This study, for the first time in Italy, analyses by WGS a strain isolated from a non-pregnant adult affected by Meningitis and without common risk factors. The strain was classified as a serotype II (SS2), sequence type ST569. Molecular characterization evidenced the presence of resistance genes to tetracycline and macrolide ((M) and (A)) and several virulence genes coding for adhesion and immune evasion factors (, family, family, , family, family and ), toxins (cfa/, family), pro-inflammatory factors (), and two homologous genes that contributed to bacterial escape from the host immune system (, ). SNP analysis showed 18 different alleles, with 9 missense SNP mutations related to genes involved in cellular metabolism (, , , and ), virulence ( and ) and antimicrobial resistance ( and ). SNPs in and genes might reduce susceptibility to drugs. The SNP analysis highlighted the presence of mutations conferring pathogenicity to the strain. The evidence in this study could explain the development of Meningitis in a healthy patient. This case highlights the importance of using molecular methods to characterize the complete genome of a bacterial species that could seriously affect human health.